Fl-DIBO |
カタログ番号GC65888
One-Click Copy Product Info
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Fl-DIBO (蛍光発生ジベンゾシクロオクチン) は、アジド化合物に対する選択的で高感度な蛍光プローブです。 Fl-DIBO はアジド化合物と急速に反応して、最大発光波長 469 nm、励起波長 363 nm の新しい高度な蛍光生成物を形成することができます。 Fl-DIBO は、バックグラウンド信号の干渉を検出することなく、ジアゾタグ付きタンパク質を標識するために使用できます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1407523-31-2
Sample solution is provided at 25 µL, 10mM.
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Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling diazo-labeled proteins[2][3]:
1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature.
2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h.
3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with Λex=365 nm, Λem=480 nm.
| Cas No. | 1407523-31-2 | SDF | |
| Formula | C19H12O3 | M.Wt | 288.3 |
| 溶解度 | Storage | Store at 2-8°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4686 mL | 17.343 mL | 34.6861 mL |
| 5 mM | 693.7 μL | 3.4686 mL | 6.9372 mL |
| 10 mM | 346.9 μL | 1.7343 mL | 3.4686 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)