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Fluorescein-5-thiosemicarbazide

カタログ番号GD13105

Products are for research use only. Not for human use. We do not sell to patients.

Fluorescein-5-thiosemicarbazide 化学構造

Cas No.: 76863-28-0

サイズ 価格 在庫数 個数
25mg
$154.00
在庫あり
100mg
$541.00
在庫あり

Tel:(909) 407-4943 Email: sales@glpbio.com


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Sample solution is provided at 25 µL, 10mM.

Description of Fluorescein-5-thiosemicarbazide

Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives.

[1]. Zhang Y, et al. One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells. Carbohydr Res. 2011 Oct 18;346(14):2156-64.  

[2]. Hav茅 M, et al. Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide. Plant Biol (Stuttg). 2015 Sep;17(5):973-9.  

Chemical Properties of Fluorescein-5-thiosemicarbazide

Cas No. 76863-28-0 SDF
Formula C21H15N3O6 M.Wt 405.36
溶解度 DMSO : 125 mg/mL (296.61 mM; Need ultrasonic) Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Fluorescein-5-thiosemicarbazide

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.4669 mL 12.3347 mL 24.6694 mL
5 mM 0.4934 mL 2.4669 mL 4.9339 mL
10 mM 0.2467 mL 1.2335 mL 2.4669 mL
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**ストックソリューションを準備する際には、常にバイアルラベルおよび MSDS/CoA(オンラインで利用可能)で掲載された製品のロット固有分子量を使用してください。

計算

In vivo Formulation Calculator (Clear solution) of Fluorescein-5-thiosemicarbazide

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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レビュー

Review for Fluorescein-5-thiosemicarbazide

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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