β-Propiolactone |
Catalog No.GC19847
One-Click Copy Product Info
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β-Propiolactone is an inactivating agent often applied for the inactivation of viruses and preparation of viral vaccines.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 57-57-8
Sample solution is provided at 25 µL, 10mM.
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β-Propiolactone is an inactivating agent often applied for the inactivation of viruses and preparation of viral vaccines [1]. β-Propiolactone can cause irreversible alkylation of nucleic acids as well as acetylation and cross-linking involving proteins, DNA, or RNA[2]. β-Propiolactone forms adducts with nucleobases (primarily guanine), and induces extensive modifications of nucleobases, nucleosides, and synthetic peptides[2]. Low concentrations of β-Propiolactone at 1:2000 (v/v) can effectively inactivate SARS-CoV-2, resulting in the loss of viral protein content and the integrity of structural protein antigens; while the high concentration leads to the aggregation of large numbers of virus particles, which may result in insufficient inactivation and loss of antigenic activity[3]. β-Propiolactone is widely used as an internal standard to optimize the analytical conditions of gas chromatography and high-performance liquid chromatography, thereby enabling the precise determination of trace-related compounds[4].
References:
[1] Uittenbogaard J P, Zomer B, Hoogerhout P, et al. Reactions of β-propiolactone with nucleobase analogues, nucleosides, and peptides: Implications for the inactivation of viruses[J]. Journal of Biological Chemistry, 2011, 286(42): 36198-36214.
[2] Pilchová V, Prajeeth C K, Jendrny P, et al. β-Propiolactone (BPL)-inactivation of SARS-co-V-2: in vitro validation with focus on saliva from COVID-19 patients for scent dog training[J]. Journal of virological methods, 2023, 317: 114733.
[3] Gupta D, Parthasarathy H, Sah V, et al. Inactivation of SARS-CoV-2 by β-propiolactone causes aggregation of viral particles and loss of antigenic potential[J]. Virus research, 2021, 305: 198555.
[4] Mirjalili S Z, Sabourian R, Chavoshi F, et al. β-Propiolactone, its application and assay methods[J]. Analytical Methods, 2025, 17(38): 7608-7617.
| Cas No. | 57-57-8 | SDF | |
| Formula | C3H4O2 | M.Wt | 72.06 |
| Solubility | DMSO : 100 mg/mL (1387.73 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 13.8773 mL | 69.3866 mL | 138.7732 mL |
| 5 mM | 2.7755 mL | 13.8773 mL | 27.7546 mL |
| 10 mM | 1.3877 mL | 6.9387 mL | 13.8773 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)