Biotin-11-dUTP |
Catalog No.GC50503
One-Click Copy Product Info
|
Biotin-11-dUTP는 PCR, 니크 번역, cDNA 합성, 랜덤 프라이머 마킹또는 프라이머 연장 과정에서 효소적으로 비방사성으로 DNA를 라벨링하는 dTTP의 형광 대체물질이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 86303-25-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Biotin-11-dUTP는 PCR, 니크 번역, cDNA 합성, 랜덤 프라이머 마킹또는 프라이머 연장 과정에서 효소적으로 비방사성으로 DNA를 라벨링하는 dTTP의 형광 대체물질이다. Biotin-11-dUTP는 역전사효소, Taq DNA 중합효소, phi29 DNA 중합효소, exonuclease-inactive Klenow fragment, Klenow fragment 및 DNA 중합효소에 의해 DNA에 통합될 수 있다[3, 4, 5].
References:
[1] Green M R, Sambrook J. Preparation of labeled DNA, RNA, and oligonucleotide probes[J]. Cold Spring Harbor Protocols, 2022, 2022(1): pdb. top100578.
[2] Stutika C, Gogol-Döring A, Botschen L, et al. A comprehensive RNA sequencing analysis of the adeno-associated virus (AAV) type 2 transcriptome reveals novel AAV transcripts, splice variants, and derived proteins[J]. Journal of virology, 2016, 90(3): 1278-1289.
[3] Steck A L. Oligonucleotide-modified Nuclotides[J]. 2013.
[4] Bharati B K, Swetha R K, Chatterji D. Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover[J]. Gene, 2013, 528(2): 99-108.
[5] Anderson J P, Angerer B, Loeb L A. Incorporation of reporter-labeled nucleotides by DNA polymerases[J]. Biotechniques, 2005, 38(2): 257-264.
이 계획은 단지 가이드를 제공하고 필요에 따라 수정하시기 바랍니다.
1.용액 준비
(1) 작업 용액: 이 제품은 용액 형태로 제공됩니다.
노트: 실제 상황에 따라 최적의 작업농도를 조정하거나 문헌을 참고하고 구배 농도 계열을 설정합니다. 작업 용액은 즉시 준비하고 사용해야 합니다.
2. Biotin-11-dUTP를 사용한 DNA 생물소화 표지[1](문헌에서, 참고용만) (1) 프라이머로 표적 유전자 프래그먼트를 PCR로 증폭합니다.
(2) 20μM Biotin-11-dUTP, 100μM 뉴클레오시드 삼인산(dNTP) 및 0.6 단위의 Klenow 조각와 함께 37°C에서 5분 동안 증폭물을 배양합니다.
(3) 미포함된 Biotin-11-dUTP와 dNTP는 에탄올 침전에 의해 제거됩니다.
(4) 생물소화된 유전자 프래그먼트를 25μL 무균 물에 재현탁하고 250μL의 streptavidin-agarose 비드와 함께 실온에서 1시간 동안 지속적으로 회전시키고 혼합합니다. (5) 세척 및 용출분의 UV 특성에 의해 DNA 생물소화를 측정합니다. DNA 생물소화는 생물소(260, 200, 240, 289nm)와 280nm (스트렙토페닌의 경우)의 특성적 UV 흡광도를 통해 정량화되었습니다.
References:
[1] Bharati B K, Swetha R K, Chatterji D. Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover[J]. Gene, 2013, 528(2): 99-108.
| Cas No. | 86303-25-5 | SDF | |
| Canonical SMILES | [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(NC/C=C/C3=CN(C(NC3=O)=O)[C@H]4C[C@@H]([C@H](O4)COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O)=O)=O.CC[N+]([H])(CC)CC.CC[N+]([H])(CC)CC.CC[N+]([H])(CC)CC.CC[N+]([H])(CC)CC | ||
| Formula | C28H45N6O17P3S | M.Wt | 862.68 |
| Solubility | 10mM Tris-HCl buffer (supplied pre-dissolved - 1mM) to 1 mM | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1592 mL | 5.7959 mL | 11.5918 mL |
| 5 mM | 231.8 μL | 1.1592 mL | 2.3184 mL |
| 10 mM | 115.9 μL | 579.6 μL | 1.1592 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solution
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)