BSA-FITC |
Catalog No.GC26406
One-Click Copy Product Info
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BSA-FITC is a fluorescently labeled protein conjugate. BSA-FITC retains the protein-binding properties of BSA while providing a strong green fluorescent signal, with an excitation wavelength of 490-495nm and an emission wavelength of 515-520nm.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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BSA-FITC is a fluorescently labeled protein conjugate. BSA-FITC retains the protein-binding properties of BSA while providing a strong green fluorescent signal, with an excitation wavelength of 490-495nm and an emission wavelength of 515-520nm. BSA-FITC can be used in related research such as cell tracking, immunofluorescence detection, drug release studies, and autoantibody detection in clinical diagnostics[1-4].
References:
[1] Kim BS, Oh JM, Kim KS, et al. BSA-FITC-loaded microcapsules for in vivo delivery. Biomaterials. 2009 Feb;30(5):902-9.
[2] Chaikhumwang P, Kitsongsermthon J, Manopakdee K, et al. Cationic Polylactic Acid-Based Nanoparticles Improve BSA-FITC Transport Across M Cells and Engulfment by Porcine Alveolar Macrophages. AAPS PharmSciTech. 2020 May 15;21(4):134.
[3] Rekowska N, Wulf K, Koper D, et al. Influence of PEGDA Molecular Weight and Concentration on the In Vitro Release of the Model Protein BSA-FITC from Photo Crosslinked Systems. Pharmaceutics. 2023 Mar 23;15(4):1039.
[4] Ashcroft RG. Measurement of ligand binding, E-BSA-FITC. Cytometry. 1986 May;7(3):298-9.
BSA-FITC Method for Assessing Blood-Brain Barrier Permeability and Endothelial Cell Endocytosis[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Prepare a 50μg/mL working solution of BSA-FITC using Hank's Balanced Salt Solution.
2. BSA-FITC Method for Determining Blood-Brain Barrier Permeability
(1) In Vitro BBB Model Construction: Seed bEnd.3 cells at a density of 50,000 cells/cm² onto the upper surface of polyester Transwell inserts (pore size 0.4μm, diameter 6.5mm) pre-coated with rat tail collagen (50μg/mL). Change the culture medium every two days and culture for approximately 3 weeks.
(2) BSA-FITC Permeability Assay: Add 200μL of Hank's solution containing 50μg/mL BSA-FITC to the upper chamber and 1mL of Hank's solution to the lower chamber. Incubate for 1 hour.
(3) Fluorescence Measurement: Aspirate 100μL of liquid from the lower chamber and measure the fluorescence intensity using a microplate reader at an excitation wavelength of 490-495nm and an emission wavelength of 515-520nm.
(4) Permeability Calculation: Calculate the apparent permeability (Pe) using the formula: Pe (μL/min·cm²)=[20×Cₜ×Cᵢ]/[19.8×(Cᵢ-Cₜ)], where Cₜ is the BSA-FITC concentration in the lower chamber of the experimental group, and Cᵢ is the BSA-FITC concentration in the lower chamber of the cell-free Transwell insert group.
3. BSA-FITC Method for Detecting Cellular Endocytosis
(1) Cell Seeding and Treatment: Seed cells at a density of 15,000 cells/cm² onto coverslips placed in 12-well plates.
(2) BSA-FITC Incubation: Incubate cells with the BSA-FITC working solution at 37°C in the dark for 1 hour.
(3) Fixation and Staining: Wash cells with ice-cold PBS, fix with 4% paraformaldehyde for 20 minutes, permeabilize with 0.3% Triton X-100 for 30 minutes, and stain nuclei with DAPI for 1 minute.
(4) Image Acquisition and Analysis: Capture images using a fluorescence microscope and perform semi-quantitative analysis of fluorescence intensity.
Precautions:
(1) The BSA-FITC working solution must be stored and used protected from light, and the incubation process should be carried out in the dark.
(2) In the cellular endocytosis experiment, wash thoroughly after BSA-FITC incubation to remove uninternalized fluorescent labels.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Xia Y, Lu YW, Hao RJ, et al. Catalpol relieved angiotensin II-induced blood-brain barrier destruction via inhibiting the TLR4 pathway in brain endothelial cells. Pharm Biol. 2022 Dec;60(1):2210-2218.
| Cas No. | SDF | ||
| Formula | M.Wt | ||
| Solubility | Storage | Store at-20℃, at least one year (Avoid repeated freeze/thaw cycles) | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Appearance: A solution
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)