(S)-Bromoenol lactone-d7 (Synonyms: (S)BELd7)

(S)-Bromoenol lactone-d₇ ((S)-BEL-d₇) contains seven deuterium atoms at the 2, 3, 4, 5, 6, 7, and 8 positions. It is intended for use as an internal standard for the quantification of (S)-BEL by GC- or LC-mass spectrometry (MS). The phospholipases are an extensive family of lipid hydrolases that function in cell signaling, digestion, membrane remodeling, and as venom components.¹ The calcium-independent phospholipase A₂ (iPLA₂) are a PLA₂ subfamily closely associated with the release of arachidonic acid in response to physiologic stimuli. (S)-Bromoenol lactone ((S)-BEL) is an irreversible, chiral, mechanism-based inhibitor of iPLA₂β that inhibits the vasopressin-induced release of arachidonate from cultured rat aortic smooth muscle (A10) cells with an IC₅₀ value of 2 µM.² (S)-BEL is more than 1,000-fold selective for iPLA₂ versus cPLA₂, and is 10-fold selective for iPLA₂β versus iPLA₂γ.

1.Balsinde, J., Balboa, M.A., Insel, P.A., et al.Regulation and inhibition of phospholipase A2Annual Reviews of Pharmacology and Toxicology39175-189(1999) 2.Jenkins, C.M., Han, X., Mancuso, D.J., et al.Identification of calcium-independent phospholipase A2 (iPLA2)β, and not iPLA2γ, as the mediator of arginine vasopressin-induced arachidonic acid release in A-10 smooth muscle cellsThe Journal of Biological Chemisty277(36)32807-32814(2002)