>>Kits>> Inhibitor Cocktails>> Protease Inhibitor Cocktails>>Protease Inhibitor Cocktail IV (EDTA-Free, 100X in DMSO)

Protease Inhibitor Cocktail IV (EDTA-Free, 100X in DMSO)

Catalog No.GK10018

Sigma P8849와 동등한 제품으로, 히스티딘 태그 단백질의 정제에 사용되어 단백질 안정성을 증가시킵니다.

Products are for research use only. Not for human use. We do not sell to patients.

Protease Inhibitor Cocktail IV (EDTA-Free, 100X in DMSO) Chemical Structure

Size 가격 재고 수량
1mL
US$19.00
재고 있음
1mL x 10
US$130.00
재고 있음
1mL x 50
US$520.00
재고 있음
1mL x 100
US$780.00
재고 있음

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고객 리뷰

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 3 publications

Description Protocol Product Documents Related Video

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in purification of Histidine-tagged proteins to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

This protease inhibitor cocktail contains individual components, including AEBSF, Bestatin, E-64, Phosphoramidon and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and thermolysin-like proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for purification of histidine-tagged proteins. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Ingredient Summary CAS Number Targets
AEBSF.HCl Serine protease inhibitor 30827-99-7 Proteases|Serine Protease
Bestatin Aminopeptidase inhibitor 58970-76-6 Proteases|Aminopeptidase
E-64 Cysteine protease inhibitor,irriversible 66701-25-5 Proteases|Cathepsin
Pepstatin A Aspartic proteinases inhibitor 26305-03-3 Proteases|Other Proteases
Phosphoramidon Disodium Salt Metalloproteinase inhibitor 164204-38-0

리뷰

Review for Protease Inhibitor Cocktail IV (EDTA-Free, 100X in DMSO)

Average Rating: 5 ★★★★★ (Based on Reviews and 7 reference(s) in Google Scholar.)

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Review for Protease Inhibitor Cocktail IV (EDTA-Free, 100X in DMSO)

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