Colestyramine (Cholestyramine resin) |
Catalog No.GC32441
One-Click Copy Product Info
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Colestyramine(콜레스티라민 수지)(Colestyramine)은 담즙산 결합 수지이며 장내 담즙산 흡수를 억제하여 콜레스테롤로부터 담즙산 합성을 증가시킬 수 있습니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11041-12-6
Sample solution is provided at 25 µL, 10mM.
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Colestyramine (Cholestyramine) is a bile acid binding resin and can inhibit intestinal bile acid absorption which results in the increasing bile acid synthesis from cholesterol.
Colestyramine (Cholestyramine) is a bile acid binding resin and can inhibit intestinal bile acid absorption which results in the increasing bile acid synthesis from cholesterol[1]. Results reveal that GSPE treatment alone, and co-administration with Colestyramine (CHY), regulate BA, cholesterol and TG metabolism differently compare to Colestyramine (CHY) administration alone. Notably, GSPE decreases intestinal apical sodium-dependent bile acid transporter (Asbt) gene expression, while Colestyramine (CHY) significantly induces expression. Administration with GSPE or Colestyramine (CHY) robustly induces hepatic BA biosynthetic gene expression, especially cholesterol 7α-hydroxylase (Cyp7a1), compare to control, while co-administration further enhances expression. Treatment with Colestyramine (CHY) induces both intestinal and hepatic cholesterologenic gene expression, while co-administration with GSPE attenuates the Colestyramine (CHY)-inducing increase in the liver but not in the intestine. Colestyramine (CHY) also induces hepatic lipogenic gene expression, which is attenuated by co-administration with GSPE[2].
[1]. Maugeais C, et al. rHDL administration increases reverse cholesterol transport in mice, but is not additive on top of ezetimibe or cholestyramine treatment. Atherosclerosis. 2013 Jul;229(1):94-101. [2]. Rebecca M. Heidker, et al. Grape Seed Procyanidins and Cholestyramine Differentially Alter Bile Acid and Cholesterol Homeostatic Gene Expression in Mouse Intestine and Liver. PLoS One. 2016; 11(4): e0154305.
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Animal experiment: |
Mice are purchased at 7 weeks of age and allowed to acclimate for one week. At 8-weeks of age the mice are given either a control or a 2% Cholestyramine-supplementing diet for 4 weeks (n=18 per group). Body weight for each mouse is recorded weekly. After 4 weeks, the mice in each group are randomly assigned to one of two treatment groups and orally gavaged with either vehicle (water) or GSPE (250 mg/kg) and terminated 14 hours later (n=9 per experimental group). The four treatment groups are as follows: 1. CON: Control diet for 4 weeks following by oral gavage with vehicle (water) for 14 hrs; 2. GSPE: Control diet for 4 weeks following by oral gavage with 250 mg/kg GSPE for 14 hrs; 3.Cholestyramine 2% Cholestyramine-supplementing diet for 4 weeks following by oral gavage with vehicle for 14 hrs; and 4. Cholestyramine+GSPE: 2% cholestyramine-supplementing diet for 4 weeks following by oral gavage with 250 mg/kg GSPE for 14 hrs. Blood is collected from the orbital plexus under isoflurane anesthesia, and intestines and livers are snap-frozen in liquid nitrogen and stored at -80°C until use. At the start of the 14 hr experiment mice are placed into clean cages, and feces are manually collected at the end of the study[2]. |
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References: [1]. Maugeais C, et al. rHDL administration increases reverse cholesterol transport in mice, but is not additive on top of ezetimibe or cholestyramine treatment. Atherosclerosis. 2013 Jul;229(1):94-101. |
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| Cas No. | 11041-12-6 | SDF | |
| Canonical SMILES | C[N+](C)(C)CC1=CC=C(C(CC(C)C2=CC=CC=C2)CC)C=C1.CC(CC)C.[Cl-].[n] | ||
| Formula | C27H47N | M.Wt | 385.67 |
| Solubility | DMSO : < 1 mg/mL (insoluble or slightly soluble);Water : < 0.1 mg/mL (insoluble) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5929 mL | 12.9645 mL | 25.9289 mL |
| 5 mM | 518.6 μL | 2.5929 mL | 5.1858 mL |
| 10 mM | 259.3 μL | 1.2964 mL | 2.5929 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)