AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH₂ |
| Catalog No.GA20705 |
Specific substrate for cathepsin D (Km = 53 µM, kcat = 7.28 s?¹, kcat/Km = 0.14 µM?¹s?¹) with a single cathepsin cleavage site between the two Phe residues. It is flanked by an N-terminal AMCA-fluorophore (7-amino-4-methylcoumarin-3-acetic acid) and a C-terminal biotin moiety. After enzymatic hydrolysis the biotin-conjugated cleavage product and undigested substrate AMCA-EEKPISFFRLGK(Biotinyl)-amide can be removed with streptavidin-coated magnetic beads. The remaining fluorescent peptide in solution represents the amount of digested substrate. The versatility of this cathepsin D 'digest and pull down assay' is applicable for routine assays.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 851231-19-1
Sample solution is provided at 25 µL, 10mM.
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Specific substrate for cathepsin D (Km = 53 µM, kcat = 7.28 s¹, kcat/Km = 0.14 µM¹s¹) with a single cathepsin cleavage site between the two Phe residues. It is flanked by an N-terminal AMCA-fluorophore (7-amino-4-methylcoumarin-3
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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