Bz-Nle-Lys-Arg-Arg-AMC (Synonyms: Benzoyl-Nle-Lys-Arg-Arg-AMC, Benzoyl-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin, Bz-Nle-Lys-Arg-Arg-AMC) |
Catalog No.GA21221 |
Bz-Nle-Lys-Arg-Arg-AMC is a fluorogenic tetra-peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 863975-32-0
Sample solution is provided at 25 µL, 10mM.
Bz-Nle-Lys-Arg-Arg-AMC is a fluorogenic tetra-peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3), dengue virus (DV) NS2B/3 serine proteases, and Zika virus (ZIKV) NS2B/NS3 serine proteases [1,2,3].
After enzymatic hydrolysis by YNS2B/NS3 serine proteases, Bz-Nle-Lys-Arg-Arg-AMC releases 7-amino-4-methylcoumarin (AMC), whose fluorescence can be used to determine the activity of YNS2B/NS3 serine proteases. AMC exhibits excitation/emission maxima at 340-360/440-460 nm, respectively.
References:
[1]. Ulanday GE, Okamoto K, Morita K. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. Tropical Medicine and Health. 2016 Dec;44:1-0.
[2]. Loehr K, Knox JE, Phong WY, Ma NL, Yin Z, Sampath A, Patel SJ, Wang WL, Chan WL, Rao KR, Wang G. Yellow fever virus NS3 protease: peptide-inhibition studies. Journal of general virology. 2007 Aug;88(8):2223-7.
[3]. Lee H, Ren J, Nocadello S, Rice AJ, Ojeda I, Light S, Minasov G, Vargas J, Nagarathnam D, Anderson WF, Johnson ME. Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from Zika virus. Antiviral research. 2017 Mar 1;139:49-58.
Average Rating: 5
(Based on Reviews and 37 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *