PolQi2 |
Catalog No.GC71127
One-Click Copy Product Info
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PolQi2을 억제 Pol θ의 helicase 활동이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2565638-16-4
Sample solution is provided at 25 µL, 10mM.
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PolQi2 is a Polθ inhibitor that targets and inhibits alt-EJ (alternative end-joining) repair by inhibiting the helicase domain at the N-terminus of Polθ [1]. PolQi2 can target DNA repair pathways to promote homology-directed repair (HDR) by regulating DNA polymerase Polθ[2]. PolQi2 treatment, combined with AZD7648, elevated HDR levels and inhibited AZD7648-induced kilobase-scale deletions across multiple loci and cell types [3]. PolQi2, combined with DNA-dependent protein-kinase (DNA-PK) can reduce insertions and deletions (indels) and improve the accuracy of various leading editing systems[4].
References:
[1] Wimberger S, Akrap N, Firth M, et al. Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing[J]. Nature communications, 2023, 14(1): 4761.
[2] Fast L, Omar M, Kanis P, et al. Search-and-remove genome editing allows selection of cells by DNA sequence[J]. Nature Communications, 2025.
[3] Cullot G, Aird E J, Schlapansky M F, et al. Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations[J]. Nature Biotechnology, 2025, 43(11): 1778-1782.
[4] Dacquay L C, Antoniou P, Mentani A, et al. Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems[J]. Nature Communications, 2025, 16(1): 4290.
| Cas No. | 2565638-16-4 | SDF | |
| Formula | C21H16ClN5O3S | M.Wt | 453.9 |
| Solubility | DMSO : 100 mg/mL (220.31 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2031 mL | 11.0156 mL | 22.0313 mL |
| 5 mM | 440.6 μL | 2.2031 mL | 4.4063 mL |
| 10 mM | 220.3 μL | 1.1016 mL | 2.2031 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)