Cycloheximide (Synonyms: Naramycin A; Actidione; 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide) |
رقم الكتالوجGC17198 |
Cycloheximide is an antibiotic that inhibits protein synthesis at the translation level, acting exclusively on cytoplasmic (80s) ribosomes of eukaryotes.
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Cas No.: 66-81-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
Hepatocyte |
Preparation Method |
Hepatocyte suspensions(2 x 106 cells/ml) were incubated in polycarbonate flasks, at 37℃, under a constant stream of 95% O2- 5% CO2, with constant shaking (72 cycles/min), for 15 min before addition of cycloheximide. |
Reaction Conditions |
Hepatocytes were treated with a range of concentrations of cycloheximide from1 x 10-7 to 5 x 10-3 M). |
Applications |
Low, non-toxic dose of cycloheximide provides reasonable assurance that protein synthetic ability could be perturbed without causing undue alterations in other biochemical functions of the cell. A nontoxic dose of cycloheximide (1 μM) inhibited termination to a greater extent than other translational steps. This effect showed a dose-dependent manner. |
Animal experiment [2]: | |
Animal models |
Male Sprague-Dawley rats,weighing between 160-230 g |
Preparation Method |
Mice were maintained on Purina Chow and water ad libitum. Food was withdrawn from the animals the night before they were given 14C-ANIT, Cycloheximide was injected 1/2, 1, 2, 4, 8 or 24 h before ANIT administration. |
Dosage form |
2 mg/kg |
Applications |
Cycloheximide is capable of protecting against ANIT-induced hyperbilirubinemia even if given 24 h before ANIT. It became apparent that cycloheximide treatment resulted in substantially reduced amounts of ANIT-equivalents in all tissues examined, even if cycloheximide was given 24 h before ANIT. |
References: [1]. Helinek TG, et al. Initial inhibition and recovery of protein synthesis in cycloheximide-treated hepatocytes. Biochem Pharmacol. 1982 Apr 1;31(7):1219-25. [2]. Lock S, et al. Effect of cycloheximide on the distribution of alpha -naphthylisothiocyanate in rats. Exp Mol Pathol. 1974 Oct;21(2):237-45. |
Cycloheximide is an antibiotic that inhibits protein synthesis at the translation level, acting exclusively on cytoplasmic (80s) ribosomes of eukaryotes. Cycloheximide affected all the energy-dependent stages in the protein-synthesizing process. However, the initiation seems the most sensitive. Cycloheximide also affects respiration, ion uptake, amino acid biosynthesis, and DNA and RNA synthesis, effects that are probably secondary to its effect on protein synthesis.[1]
In vitro study indicated that Cycloheximide at 1 μM inhibited [3H]leucine incorporation into both cellular and secreted proteins by at least 86%, without having deleterious effects on membrane integrity as indicated by trypan blue uptake and lactate dehydrogenase release. Larger size class polysomes (7+) were increased by Cycloheximide treatment and remained increased during recovery. [2]
In vivo analysis indicated that Cycloheximide produced initial hyperactivity. This initial hyperactivity was apparent within 3 minutes after injection of the Cycloheximide. Cycloheximide affects activity by acting on the brain, and this is unrelated to its inhibition of protein synthesis. In addition, Cycloheximide’s effects on activity did not appear to be responsible for its amnesic action. However, Cycloheximide might have some other property, unrelated to inhibition of cerebral protein synthesis, that is responsible for its amnesic effect.[3]
References:
[1]. Marcos R, et al. Effect of Cycloheximide on different stages of Drosophila melanogaster. Toxicol Lett. 1982 Sep;13(1-2):105-12.
[2].Helinek TG, et al. Initial inhibition and recovery of protein synthesis in cycloheximide-treated hepatocytes. Biochem Pharmacol. 1982 Apr 1;31(7):1219-25.
[3]. Segal DS, et al. Cycloheximide: its effects on activity are dissociable from its effects on memory. Science. 1971 Apr 2;172(3978):82-4.
Cas No. | 66-81-9 | SDF | |
المرادفات | Naramycin A; Actidione; 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide | ||
Chemical Name | 4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione | ||
Canonical SMILES | CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C | ||
Formula | C15H23NO4 | M.Wt | 281.4 |
الذوبان | ≥ 100 mg/mL (355.87 mM) in DMSO, ≥ 57.6 mg/mL in EtOH, ≥ 14.05 mg/mL in Water with ultrasonic and warming | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.5537 mL | 17.7683 mL | 35.5366 mL |
5 mM | 0.7107 mL | 3.5537 mL | 7.1073 mL |
10 mM | 0.3554 mL | 1.7768 mL | 3.5537 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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