Accueil>>Signaling Pathways>> Microbiology & Virology>> Fungal>>Cycloheximide

Cycloheximide (Synonyms: Naramycin A; Actidione; 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide)

Catalog No.GC17198

Cycloheximide is an antibiotic that inhibits protein synthesis at the translation level, acting exclusively on cytoplasmic (80s) ribosomes of eukaryotes.

Products are for research use only. Not for human use. We do not sell to patients.

Cycloheximide Chemical Structure

Cas No.: 66-81-9

Taille Prix Stock Qté
10mM (in 1mL DMSO)
41,00 $US
En stock
200mg
39,00 $US
En stock
500mg
54,00 $US
En stock
1g
80,00 $US
En stock
5g
262,00 $US
En stock

Tel:(909) 407-4943 Email: sales@glpbio.com

Avis des clients

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 13 publications

Product Documents

Quality Control & SDS

View current batch:

Protocol

Cell experiment [1]:

Cell lines

Hepatocyte

Preparation Method

Hepatocyte suspensions(2 x 106 cells/ml) were incubated in polycarbonate flasks, at 37℃, under a constant stream of 95% O2- 5% CO2, with constant shaking (72 cycles/min), for 15 min before addition of cycloheximide.

Reaction Conditions

Hepatocytes were treated with a range of concentrations of cycloheximide from1 x 10-7 to 5 x 10-3 M).

Applications

Low, non-toxic dose of cycloheximide provides reasonable assurance that protein synthetic ability could be perturbed without causing undue alterations in other biochemical functions of the cell. A nontoxic dose of cycloheximide (1 μM) inhibited termination to a greater extent than other translational steps. This effect showed a dose-dependent manner.

Animal experiment [2]:

Animal models

Male Sprague-Dawley rats,weighing between 160-230 g

Preparation Method

Mice were maintained on Purina Chow and water ad libitum. Food was withdrawn from the animals the night before they were given 14C-ANIT, Cycloheximide was injected 1/2, 1, 2, 4, 8 or 24 h before ANIT administration.

Dosage form

2 mg/kg

Applications

Cycloheximide is capable of protecting against ANIT-induced hyperbilirubinemia even if given 24 h before ANIT. It became apparent that cycloheximide treatment resulted in substantially reduced amounts of ANIT-equivalents in all tissues examined, even if cycloheximide was given 24 h before ANIT.

References:

[1]. Helinek TG, et al. Initial inhibition and recovery of protein synthesis in cycloheximide-treated hepatocytes. Biochem Pharmacol. 1982 Apr 1;31(7):1219-25.

[2]. Lock S, et al. Effect of cycloheximide on the distribution of alpha -naphthylisothiocyanate in rats. Exp Mol Pathol. 1974 Oct;21(2):237-45.

Background

Cycloheximide is an antibiotic that inhibits protein synthesis at the translation level, acting exclusively on cytoplasmic (80s) ribosomes of eukaryotes. Cycloheximide affected all the energy-dependent stages in the protein-synthesizing process. However, the initiation seems the most sensitive. Cycloheximide also affects respiration, ion uptake, amino acid biosynthesis, and DNA and RNA synthesis, effects that are probably secondary to its effect on protein synthesis.[1]

In vitro study indicated that Cycloheximide at 1 μM inhibited [3H]leucine incorporation into both cellular and secreted proteins by at least 86%, without having deleterious effects on membrane integrity as indicated by trypan blue uptake and lactate dehydrogenase release. Larger size class polysomes (7+) were increased by Cycloheximide treatment and remained increased during recovery. [2]

In vivo analysis indicated that Cycloheximide produced initial hyperactivity. This initial hyperactivity was apparent within 3 minutes after injection of the CycloheximideCycloheximide affects activity by acting on the brain, and this is unrelated to its inhibition of protein synthesis. In addition, Cycloheximide’s effects on activity did not appear to be responsible for its amnesic action. However, Cycloheximide might have some other property, unrelated to inhibition of cerebral protein synthesis, that is responsible for its amnesic effect.[3]

References:
[1]. Marcos R, et al. Effect of Cycloheximide on different stages of Drosophila melanogaster. Toxicol Lett. 1982 Sep;13(1-2):105-12.
[2].Helinek TG, et al. Initial inhibition and recovery of protein synthesis in cycloheximide-treated hepatocytes. Biochem Pharmacol. 1982 Apr 1;31(7):1219-25.
[3]. Segal DS, et al. Cycloheximide: its effects on activity are dissociable from its effects on memory. Science. 1971 Apr 2;172(3978):82-4.

Chemical Properties

Cas No. 66-81-9 SDF
Synonymes Naramycin A; Actidione; 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide
Chemical Name 4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione
Canonical SMILES CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C
Formula C15H23NO4 M.Wt 281.4
Solubility ≥ 100 mg/mL (355.87 mM) in DMSO, ≥ 57.6 mg/mL in EtOH, ≥ 14.05 mg/mL in Water with ultrasonic and warming Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.5537 mL 17.7683 mL 35.5366 mL
5 mM 0.7107 mL 3.5537 mL 7.1073 mL
10 mM 0.3554 mL 1.7768 mL 3.5537 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Avis

Review for Cycloheximide

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for Cycloheximide

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.