Hecubine (Synonyms: Hecubine) |
رقم الكتالوجGF11023
One-Click Copy Product Info
|
Hecubine is a natural aspidosperma-type alkaloid found in E. officinalis, with anti-neuroinflammation properties.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62874-52-6
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Hecubine is a natural aspidosperma-type alkaloid found in E. officinalis, with anti-neuroinflammation properties [1]. Hecubine can alleviate neuroinflammation and oxidative stress by modulating the Nrf2 and TLR4 signaling pathways [2]. Hecubine has been widely used to inhibit the growth of various types of cancer cells[3].
In vitro, Hecubine pre-treatment (25μM) for 1 hour can effectively reduce the production of NO and PGE2 and the expression of iNOS and COX-2 in BV2 cells stimulated by Lipopolysaccharide (LPS) [4].
References:
[1] Gonzalez C G, Martinez J. Phytochemistry of Ervatamia coronaria Stapf.(II). Hecubine and voaphylline: two alkaloids present in leaves[J]. Rev. Cubana Farmac, 1976, 10: 45-54.
[2] Tan X, Liu H, Liang F, et al. Zebrafish as a multimodal platforms for anti-inflammatory phytomedicine discovery and translation[J]. Frontiers in Pharmacology, 2026, 17: 1775366.
[3] Naidoo C M, Naidoo Y, Dewir Y H, et al. Major bioactive alkaloids and biological activities of Tabernaemontana species (Apocynaceae)[J]. Plants, 2021, 10(2): 313.
[4] Li L, He Y L, Xu N, et al. A natural small molecule aspidosperma-type alkaloid, hecubine, as a new TREM2 activator for alleviating lipopolysaccharide-induced neuroinflammation in vitro and in vivo[J]. Redox biology, 2024, 70: 103057.
| Cell experiment [1]: | |
Cell lines | BV2 cells |
Preparation Method | BV2 cells were cultivated in 24-well plate at a density of 4×105 cells/ml in DMEM medium supplemented with 10% fetal bovine serum (FBS), phosphate-buffered saline (PBS), and 1% penicillin/streptomycin. Cells were treated with different concentrations of the Hecubine (0, 6, 12, and 25µM) for 1h prior to LPS stimulation (800ng/ml). The levels of NO, PGE2, and IL-6 in cells were determined. |
Reaction Conditions | 0, 6, 12, and 25µM; 1h |
Applications | Hecubine treatment reduced the levels of NO, PGE2, and IL-6 in BV2 cells exposed to LPS in a dose-dependent manner. |
References: | |
| Cas No. | 62874-52-6 | SDF | |
| المرادفات | Hecubine | ||
| Formula | C20H26N2O | M.Wt | 310.433 |
| الذوبان | Storage | Store at 2-8°C,protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2213 mL | 16.1065 mL | 32.2131 mL |
| 5 mM | 644.3 μL | 3.2213 mL | 6.4426 mL |
| 10 mM | 322.1 μL | 1.6107 mL | 3.2213 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)