Patulin (Synonyms: Clairformin, Expansin, Mycoin C, NSC 8120, NSC 32951) |
رقم الكتالوجGC40483
One-Click Copy Product Info
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الباتولين (تيرينين) هو سم فطري تنتجه الفطريات بما في ذلك أنواع الرشاشيات والبنسيليوم والبيسوكلاميس ، ويُشتبه في أنه مسبب للتخثر ، ومسبب للطفرات ، وماسخ للخلايا
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 149-29-1
Sample solution is provided at 25 µL, 10mM.
Patulin is a toxic polyphenolic lactolactone [1]. Patulin reacts with protein sulfhydryl groups, triggering cellular oxidative stress and p53-mediated DNA damage, which damages the intestinal epithelial barrier [2]. Patulin has teratogenic, genotoxic, and neurotoxic properties [3-4].
In HL-60 cells, after Patulin (0-10μM; 6h) significantly decreased cell viability [5]. In HepG2 cells, Patulin (0.5-2μM; 24h) induces autophagy in cells via the ROS-Akt1-MTOR pathway [6].
In CF-1 mice, Patulin (1.0-3.75mg/kg; ip; single injection) induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney [7]. In B16F10 cell xenograft mouse model, Patulin (5-50μg/kg; ip; 20d) significantly induced tumor regression [8].
References:
[1]. Puel O, Galtier P, Oswald I P. Biosynthesis and toxicological effects of patulin[J]. Toxins, 2010, 2(4): 613-631.
[2]. Saxena N, Ansari K M, Kumar R, et al. Patulin causes DNA damage leading to cell cycle arrest and apoptosis through modulation of Bax, p53 and p21/WAF1 proteins in skin of mice[J]. Toxicology and applied pharmacology, 2009, 234(2): 192-201.
[3]. Glaser N, Stopper H. Patulin: Mechanism of genotoxicity[J]. Food and Chemical Toxicology, 2012, 50(5): 1796-1801.
[4]. Lei W L, Li Y Y, Hou Y, et al. Toxic effects of patulin on mouse oocytes and its possible mechanisms[J]. Toxicology, 2021, 464: 153013.
[5]. Wu T S, Liao Y C, Yu F Y, et al. Mechanism of patulin-induced apoptosis in human leukemia cells (HL-60)[J]. Toxicology letters, 2008, 183(1-3): 105-111.
[6]. Yang G, Bai Y, Wu X, et al. Patulin induced ROS-dependent autophagic cell death in Human Hepatoma G2 cells[J]. Chemico-Biological Interactions, 2018, 288: 24-31.
[7]. de Melo F T, de Oliveira I M, Greggio S, et al. DNA damage in organs of mice treated acutely with patulin, a known mycotoxin[J]. Food and Chemical Toxicology, 2012, 50(10): 3548-3555.
[8]. Boussabbeh M, Ben Salem I, Rjiba-Touati K, et al. The potential effect of patulin on mice bearing melanoma cells: an anti-tumour or carcinogenic effect?[J]. Tumor Biology, 2016, 37(5): 6285-6295.
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 (1 × 105 cells) were seeded in 96-well plates, treated with vehicle (15% ethanol in 0.01M PBS) alone or various concentrations (final concentration 0-10μM) of Patulin at the designated time. |
Reaction Conditions | 0-10μM; 6h |
Applications | Patulin treatment significantly decreased cell viability. |
| Animal experiment [2]: | |
Animal models | CF-1 mice |
Preparation Method | The experimental design involved nine groups of 10 mice, housed individually in cages. Mice received a single intraperitoneal (i.p.) dose of 1.0, 2.5, or 3.75mg/kg body weight (BW) of Patulin. Patulin stock solution was dissolved in DMSO and then diluted with saline to a final DMSO concentration below 0.1%. Mice were divided into the following groups: Group 1: Control group, administered with 0.1% DMSO in saline (5mL/kg BW). Group 2: Positive control group, administered with MMS (40mg/kg BW). Groups 3-5: Patulin was administered intraperitoneally at the following doses: Group 3, 1.0mg/kg BW; Group 4, 2.5mg/kg BW; Group 5, 3.75mg/kg BW. Group 6: N-ac (1840μmol/kg BW) was administered 3 hours prior to administration of 0.1% DMSO (5mL/kg BW). Groups 7-9 were intraperitoneally injected with Patulin at the following doses: 1.0mg/kg body weight for Group 7; 2.5mg/kg body weight for Group 8; and 3.75mg/kg body weight for Group 9. All animals in Groups 7-9 were given 300mg/kg of N-ac 3 hours before Patulin. |
Dosage form | 1.0-3.75mg/kg; ip; single injection |
Applications | Patulin induced DNA strand breaks, decreased GSH levels, and increased lipid peroxidation in the brain, liver, and kidney. |
References: | |
| Cas No. | 149-29-1 | SDF | |
| المرادفات | Clairformin, Expansin, Mycoin C, NSC 8120, NSC 32951 | ||
| Chemical Name | 4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one | ||
| Canonical SMILES | O=C1OC2=CCOC(O)C2=C1 | ||
| Formula | C7H6O4 | M.Wt | 154.1 |
| الذوبان | 13mg/mL in ethanol, 10mg/mL in DMSO, 20mg/mL in DMF | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.4893 mL | 32.4465 mL | 64.8929 mL |
| 5 mM | 1.2979 mL | 6.4893 mL | 12.9786 mL |
| 10 mM | 648.9 μL | 3.2446 mL | 6.4893 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 17 reference(s) in Google Scholar.)