Hexa His tag peptide (Synonyms: H-His-His-His-His-His-His-OH) |
| Catalog No.GP10154 |
The Hexa His tag peptide est un peptide court composé de six résidus d'histidine consécutifs, qui sert également d'étiquette de purification pour les protéines de fusion. The Hexa His tag peptide peut chélater des ions métalliques tels que le nickel et le cobalt. Les protéines recombinantes peuvent être efficacement purifiées par chromatographie d'affinité sur ions métalliques immobilisés.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 64134-30-1
Sample solution is provided at 25 µL, 10mM.
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The Hexa His tag peptide est un peptide court composé de six résidus d'histidine consécutifs, qui sert également d'étiquette de purification pour les protéines de fusion. The Hexa His tag peptide peut chélater des ions métalliques tels que le nickel et le cobalt. Les protéines recombinantes peuvent être efficacement purifiées par chromatographie d'affinité sur ions métalliques immobilisés[1]. The Hexa His tag peptide est couramment utilisé pour réparer des mimétiques protéiques destinés aux nanocristaux[2].
References:
[1] Schmidt TG, Koepke J, Frank R, et al. Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J Mol Biol. 1996 Feb 9;255(5):753-66.
[2] Zhou W, Baneyx F. Biofabrication of ZnS:Mn luminescent nanocrystals using histidine, hexahistidine, and His-tagged proteins: a comparison study. Biochem Eng J. 2014 Aug 15;89:28-32.
| Cas No. | 64134-30-1 | SDF | |
| Synonymes | H-His-His-His-His-His-His-OH | ||
| Chemical Name | Hexa His | ||
| Canonical SMILES | C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N | ||
| Formula | C36H44N18O7 | M.Wt | 840.85 |
| Solubility | ≥84.1mg/mL in DMSO, ≥123.4 mg/mL in EtOH with ultrasonic, ≥67.5 mg/mL in Water | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1893 mL | 5.9464 mL | 11.8927 mL |
| 5 mM | 237.9 μL | 1.1893 mL | 2.3785 mL |
| 10 mM | 118.9 μL | 594.6 μL | 1.1893 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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