246C10 |
カタログ番号GC63985
One-Click Copy Product Info
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246C10はイオン化可能なカチオン脂質である(pKa = 6.75)。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2635329-26-7
Sample solution is provided at 25 µL, 10mM.
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246C10はイオン化可能なカチオン脂質である(pKa = 6.75)[1]。246C10は、ジオレオイルホスファチジルエタノールアミン（DOPE）、コレステロールとC16-PEG2000セラミド（PEG-脂質）などの脂質と併用でき、よって細胞にRNA治療薬を標的送達するための脂質ナノ粒子（LNP）を生成する[2, 3]。246C10を、mRNAエンコーディングルシフェラーゼ（mFLuc）とDOPE、コレステロール、PEG-脂質、マンノース-PEG脂質とともにLNPとして調製した。ApoEノックアウトマウスに静脈内注射した結果、肺のルシフェラーゼ発見を著しく増やした[4]。Cas9とsgRNAを負荷した246C10 LNPを使い、マウス肺におけるATの標的遺伝子を編集することで、オフターゲット効果、肺毒性、大きい抗Cas9免疫応答を導入せずに、マウスアンチトロンビン（AT）の生産を阻害した[5]。
References:
[1] Tang X, Zhang Y, Han X. Ionizable Lipid Nanoparticles for mRNA Delivery[J]. Advanced NanoBiomed Research, 2023, 3(8): 2300006.
[2] Jin H, Jeong M, Lee G, et al. Engineered lipid nanoparticles for the treatment of pulmonary fibrosis by regulating epithelial‐mesenchymal transition in the lungs[J]. Advanced Functional Materials, 2023, 33(7): 2209432.
[3] Wu S, Lin L, Shi L, et al. An overview of lipid constituents in lipid nanoparticle mRNA delivery systems[J]. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2024, 16(4): e1978.
[4] Kim M, Jeong M, Hur S, et al. Engineered ionizable lipid nanoparticles for targeted delivery of RNA therapeutics into different types of cells in the liver[J]. Science advances, 2021, 7(9): eabf4398.
[5] Liu Z, Li Z, Li B. Nonviral delivery of CRISPR/Cas systems in mRNA format[J]. Advanced NanoBiomed Research, 2022, 2(11): 2200082.
| Cas No. | 2635329-26-7 | SDF | |
| Formula | C58H120N4O4 | M.Wt | 937.6 |
| 溶解度 | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0666 mL | 5.3328 mL | 10.6655 mL |
| 5 mM | 213.3 μL | 1.0666 mL | 2.1331 mL |
| 10 mM | 106.7 μL | 533.3 μL | 1.0666 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)