246C10 |
Catalog No.GC63985
One-Click Copy Product Info
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246C10은 이온화 가능한 양이온성 지질이다 (pKa=6.75).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2635329-26-7
Sample solution is provided at 25 µL, 10mM.
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246C10은 이온화 가능한 양이온성 지질이다 (pKa=6.75). 246C10은 dioleylphosphatidylethanolamine (DOPE), 콜레스테롤, C16-PEG2000 세라마이드 (PEG-지질)와 같은 지질과 결합하여 RNA 치료제를 세포로 대상적으로 전달하기 위한 지질 나노입자 (LNPs)을 생성할 수 있다[2, 3]. 246C10은 루시페라제(mFLuc)를 인코딩하는 mRNA, DOPE, 콜레스테롤, PEG-지질과 mannose-PEG 지질과 함께 LNPs로 구성되었다. ApoE 결함 쥐에 지혈로 주입되었을 때 간에서 루시페라제 표현을 현저하게 증가시켰다[4]. Cas9와 sgRNA를 로드한 246C10 LNPs를 사용하여 쥐 간에서 AT를 대상으로 유전자 편집을 실시함으로써 쥐 트롬빈 방지 효소 (AT)의 생산을 억제하는 동시에 오프-타겟 효과, 간 독성 및 강한 항-Cas9 면역 반응을 유발하지 않았다[5].
References:
[1] Tang X, Zhang Y, Han X. Ionizable Lipid Nanoparticles for mRNA Delivery[J]. Advanced NanoBiomed Research, 2023, 3(8): 2300006.
[2] Jin H, Jeong M, Lee G, et al. Engineered lipid nanoparticles for the treatment of pulmonary fibrosis by regulating epithelial‐mesenchymal transition in the lungs[J]. Advanced Functional Materials, 2023, 33(7): 2209432.
[3] Wu S, Lin L, Shi L, et al. An overview of lipid constituents in lipid nanoparticle mRNA delivery systems[J]. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2024, 16(4): e1978.
[4] Kim M, Jeong M, Hur S, et al. Engineered ionizable lipid nanoparticles for targeted delivery of RNA therapeutics into different types of cells in the liver[J]. Science advances, 2021, 7(9): eabf4398.
[5] Liu Z, Li Z, Li B. Nonviral delivery of CRISPR/Cas systems in mRNA format[J]. Advanced NanoBiomed Research, 2022, 2(11): 2200082.
| Cas No. | 2635329-26-7 | SDF | |
| Formula | C58H120N4O4 | M.Wt | 937.6 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0666 mL | 5.3328 mL | 10.6655 mL |
| 5 mM | 213.3 μL | 1.0666 mL | 2.1331 mL |
| 10 mM | 106.7 μL | 533.3 μL | 1.0666 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)