β-Asarone (Synonyms: cis-Asarone) |
| カタログ番号GC40574 |
β-Asaroneはイチョウの根茎の主成分であり、中枢神経系（CNS）に顕著な薬理作用がある。
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Cas No.: 5273-86-9
Sample solution is provided at 25 µL, 10mM.
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β-Asaroneはイチョウの根茎の主成分であり、中枢神経系（CNS）に顕著な薬理作用がある[1-3]。β-Asaronは血液脳関門（BBB）を通過することができる[4]。β-AsaronはMAO-A(IC50=142±18µM)とMAO-B（IC50=362±43µM）を阻害できる。
β-Asarone (30 µg/mL)はPC12細胞におけるプロテインキナーゼA通路を活性化することでCREBをリン酸化し、そこで、神経成長因子（NGF）が誘導したニューロン分化を増加する[6]。β-AsaroneはLPSが活性化したBV-2ミクログリアおよびうつ病に対して神経の活性の保護がある[2,7-8]。
β-Asarone(20および30 mg/kg/d)はラットの脳虚血と再灌流後の神経行動機能の神経学的結果を改善することができる[9]。β-Asaroneはβ-アミロイド海馬を注射したラットの神経細胞のアポトーシスを阻害することにより、認知機能を改善する [10]。
References:
[1]. Cho J, Kim YH, Kong JY, Yang CH, Park CG. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sciences. 2002 Jun 21;71(5):591-9.
[2]. Chen YZ, Wang QW, Liang Y, Fang YQ. Protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate. Zhong yao cai= Zhongyaocai= Journal of Chinese Medicinal Materials. 2007 Apr 1;30(4):436-9.
[3]. Fang YQ, Fang RM, Fang GL, Jiang Y, Fu SY. Effects of beta-asarone on expression of c-fos in kindling epilepsy rat brain. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica. 2008 Mar 1;33(5):534-6.
[4]. Fang YQ, Wei G. To analyze if the Rhizoma Acori Tatarimowii naph-tha can go through the BBB or not with GC-MS. Zhong Yao Xin Yao Yu: Ling Chuang Yao Li. 2002;3:181-2.
[5]. Tao G, Irie Y, Li DJ, Keung WM. Eugenol and its structural analogs inhibit monoamine oxidase A and exhibit antidepressant-like activity. Bioorganic & medicinal chemistry. 2005 Aug 1;13(15):4777-88.
[6]. Lam KY, Chen J, Lam CT, Wu Q, Yao P, Dong TT, Lin H, Tsim KW. Asarone from Acori Tatarinowii Rhizoma potentiates the nerve growth factor-induced neuronal differentiation in cultured PC12 cells: a signaling mediated by protein kinase A. PLoS One. 2016 Sep 29;11(9):e0163337.
[7]. Li C, Xing G, Dong M, Zhou L, Li J, Wang G, Zou D, Wang R, Liu J, Niu Y. Beta-asarone protection against beta-amyloid-induced neurotoxicity in PC12 cells via JNK signaling and modulation of Bcl-2 family proteins. European Journal of Pharmacology. 2010 Jun 10;635(1-3):96-102.
[8].Lim HW, Kumar H, Kim BW, More SV, Kim IW, Park JI, Park SY, Kim SK, Choi DK. β-Asarone (cis-2, 4, 5-trimethoxy-1-allyl phenyl), attenuates pro-inflammatory mediators by inhibiting NF-κB signaling and the JNK pathway in LPS activated BV-2 microglia cells. Food and chemical toxicology. 2014 Oct 1;72:265-72.
[9]. Yang YX, Chen YT, Zhou XJ, Hong CL, Li CY, Guo JY. Beta-asarone, a major component of Acorus tatarinowii Schott, attenuates focal cerebral ischemia induced by middle cerebral artery occlusion in rats. BMC Complementary and Alternative Medicine. 2013 Dec;13:1-6.
[10]. Geng Y, Li C, Liu J, Xing G, Zhou L, Dong M, Li X, Niu Y. Beta-asarone improves cognitive function by suppressing neuronal apoptosis in the beta-amyloid hippocampus injection rats. Biological and Pharmaceutical Bulletin. 2010 May 1;33(5):836-43.
| 細胞実験[1]: | |
細胞株 | 自発的不死化ラットシュワン細胞株RSC96 |
準備方法 | RSC96 細胞を0、5、10、20µMのβ-Asaroneで2、4、6日間処理する。細胞は0.25%トリプシンで消化され、60µg/mLのプロテアーゼKを含んでいるPBSに再懸濁させ、60°Cの温度で6時間かかる。 |
反応条件 | 0, 5, 10, and 20 µM for 2, 4, and 6 d |
アプリケーション | β-Asaroneが培養した細胞は対照群より成長が速い。3 つの濃度の中で、10 μM β-アサロンが同じ培養時点での細胞増殖に対して最も強い促進効果を示めす。 |
| 動物実験 [2]: | |
動物モデル | 成年のウィスターラット |
準備方法 | 動物を5つの群に分け、1群あたり10匹のラットにする。第1群はシャムオペレーション（SHAM）とする。第2群は虚血群（MCAO）である。I群とII群はそれぞれ蒸留水を30日間経口投与する。群III（β-Asarone-10）、群IV（β-Asarone-20）、群V（β-Asarone-30）はβ-Asarone（それぞれ10、20、30 mg/kg/日）を経口投与する。30日後に中脳動脈閉塞(MCAO)は脳虚血を引き起こす。 |
投与形態 | 10、20、および 30 mg/kg/日、経口、30 日間。 |
アプリケーション | MCAO群に比べると、β-Asarone-20群とβ-Asarone-30群は行動異常の発生を顕著に阻害する。 |
References: | |
| Cas No. | 5273-86-9 | SDF | |
| 同義語 | cis-Asarone | ||
| Canonical SMILES | COC1=CC(/C=C\C)=C(OC)C=C1OC | ||
| Formula | C12H16O3 | M.Wt | 208.3 |
| 溶解度 | DMF: 16 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 11 mg/ml,Ethanol: 14 mg/ml | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.8008 mL | 24.0038 mL | 48.0077 mL |
| 5 mM | 0.9602 mL | 4.8008 mL | 9.6015 mL |
| 10 mM | 0.4801 mL | 2.4004 mL | 4.8008 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)
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