Adapalene-d3 |
カタログ番号GC46798
One-Click Copy Product Info
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アダパレンの定量化のための内部標準
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1276433-89-6
Sample solution is provided at 25 µL, 10mM.
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Adapalene-d3 is intended for use as an internal standard for the quantification of adapalene by GC- or LC-MS. Adapalene is a synthetic retinoid and an agonist of retinoic acid receptors (RARs; Kds = 1,100, 34, and 130 nM for RARα, RARβ, and RARγ, respectively).1,2,3,4,5 It inhibits growth and differentiation of sebocytes in a concentration-dependent manner in primary rat preputial cell culture. Adapalene (10 μM) completely inhibits the activity of soybean 15-lipoxygenase (15-LOX) in an enzyme assay and inhibits the 5- and 15-LOX pathways in human blood polymorphonuclear leukocytes (PMNs).2 It reduces the protein levels of toll-like receptor 2 (TLR2) and IL-10 in skin explants isolated from patients with acne and healthy controls in a concentration-dependent manner but increases the expression of the antigen-presenting protein CD1d in acne skin explants while decreasing it in control explants.3 It inhibits inflammation in rodent models of ear edema induced by arachidonic acid and carrageenan-induced paw edema.2 Adapalene (100 μM) also induces apoptosis and inhibits proliferation of CC-531, HT-29, and LoVo colon cancer cells and reduces tumor growth in a DLD-1 colon cancer nude mouse xenograft model in a dose-dependent manner.5,4 Formulations containing adapalene have been used in the treatment of acne vulgaris.
1.Kim, M.J., Ciletti, N., Michel, S., et al.The role of specific retinoid receptors in sebocyte growth and differentiation in cultureJournal of Investigative Dermatology114349-353(2000) 2.Hensby, C., Cavey, D., Bouclier, M., et al.The in vivo and in vitro anti-inflammatory activity of CD271: A new retinoid-like modulator of cell differentiationAgents Actions29(1-2)56-58(1990) 3.Tenaud, I., Khammari, A., and Dreno, B.In vitro modulation of TLR-2, CD1d and IL-10 by adapalene on normal human skin and acne inflammatory lesionsExp. Dermatol.16(6)500-506(2007) 4.Ocker, M., Herold, C., Ganslmayer, M., et al.The synthetic retinoid adapalene inhibits proliferation and induces apoptosis in colorectal cancer cells in vitroInt. J. Cancer107(3)453-459(2003) 5.Shi, X.-N., Li, H., Yao, H., et al.Adapalene inhibits the activity of cyclin-dependent kinase 2 in colorectal carcinomaMol. Med. Rep.12(5)6501-6508(2015)
| Cas No. | 1276433-89-6 | SDF | |
| Canonical SMILES | [2H]C([2H])([2H])OC1=C(C2(C[C@H](C3)C4)C[C@H]4C[C@H]3C2)C=C(C=C1)C(C=C5)=CC6=C5C=C(C(O)=O)C=C6 | ||
| Formula | C28H25D3O3 | M.Wt | 415.5 |
| 溶解度 | DMF: 5 mg/ml,DMSO: 5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4067 mL | 12.0337 mL | 24.0674 mL |
| 5 mM | 481.3 μL | 2.4067 mL | 4.8135 mL |
| 10 mM | 240.7 μL | 1.2034 mL | 2.4067 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)