5-Hydroxytryptamine (Serotonin)

Cell experiment [1]:

Cell lines

Osteoblasts

Preparation method

Osteoblasts at passage 3 were used to perform all cell studies and were divided into six groups, which were cultured in the presence of various concentrations of 5-HT : 0 M (control group) and 1 nM, 10 nM, 100 nM, 1 µM, and 10 µM (experimental groups). Primary osteoblasts were incubated with 5-HT for 2 days, and the proliferation rate was measured.

Reaction Conditions

0 M and 1 nM, 10 nM, 100 nM, 1 µM, and 10 µM; 2 days

Applications

Growth of osteoblasts treated with serotonin (1 nM-10 µM) was inhibited compared to controls. This influence was alleviated in the 10 µM and 1 µM groups (relatively high concentrations).

Animal experiment [2]:

Animal models

C57BL/6 mice

Preparation method

Twenty-five adult female C57BL/6 mice were divided into five groups. Colon tumours in mice were induced by AOM and DSS. Respectively, 10 mg/kg 5-HT was intraperitoneally injected (5-HT group), 20 mg/kg citalopram in normal saline was administered intragastrically (citalopram group), and then both 5-HT and citalopram were administered in combination (combined group). Following the procedure, the mice were sacrificed after 90 days, and their blood serum was collected to quantify 5-HT contents. The tumours were separated from each animal, and their sizes and volumes were measured.

Dosage form

10 mg/kg, i.p.

Applications

After 90 days of treatment with 10 mg/kg 5-HT, the tumour number and volume in the mice’s intestinal mucosa were 11.60 ± 1.52 and 14.35 ± 2.72 mm3, respectively. 5-HT promotes primary colon carcinogenesis in mice.

References:

[1] Dai SQ, et al. Serotonin regulates osteoblast proliferation and function in vitro. Braz J Med Biol Res. 2014 Sep;47(9):759-65.

[2] Yu H, et al. Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression. Cell Commun Signal. 2023 Apr 12;21(1):75.