(±)12(13)-EpOME MaxSpec® Standard (Synonyms: (±)12,13-EODE, Isoleukotoxin, (±)-Vernolic Acid)

(±)12(13)-EpOME is the 12,13-cis epoxide form of linoleic acid .^1,2 It is formed primarily via linoleic acid metabolism by the cytochrome P450 (CYP) isoforms CYP2J2, CYP2C8, and CYP2C9, however, CYP1A1 can contribute to (±)12(13)-EpOME production when pharmacologically induced.² (±)12(13)-EpOME (500 µM) induces mitochondrial dysfunction and cell death in renal proximal tubule epithelial cells.

(±)12(13)-EpOME MaxSpec^® standard is a quantitative grade standard of (±)12(13)-EpOME that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)12(13)-EpOME MaxSpec^® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

1.Hayakawa, M., Sugiyama, S., Takamura, T., et al.Neutrophils biosynthesize leukotoxin, 9,10-epoxy-12-octadecenoateBiochem. Biophys. Res. Commun.137(1)424-430(1986) 2.Hildreth, K., Kodani, S.D., Hammock, B.D., et al.Cytochrome P450-derived linoleic acid metabolites EpOMEs and DiHOMEs: A review of recent studiesJ. Nutr. Biochem.19108484(2020)