5-bromo-3-indolyl-β-D-Galactopyranoside (Synonyms: Bluo-Gal) |
| カタログ番号GC49877 |
5-ブロモ-3-インドリル-β-D-ガラクトピラノシド (Bluo-Gal) は、細菌 β-D-ガラクトシダーゼ活性を検出する発色基質です。
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Cas No.: 97753-82-7
Sample solution is provided at 25 µL, 10mM.
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5-Bromo-3-indolyl-β-D-galactopyranoside (Bluo-Gal) is a cell-permeable chromogenic substrate for β-galactosidase.1 Upon enzymatic hydrolysis, it generates an indigo-colored precipitate that can be used to measure β-galactosidase activity. Bluo-Gal has been used to detect the expression of β-galactosidase in muscle from transgenic mice expressing E. coli lacZ, the gene that encodes β-galactosidase.
1.Gioglio, L., de Angelis, M.G.C., Boratto, R., et al.An improved method for β-galactosidase activity detection on muscle tissue. A light and electron microscopic studyAnn. Anat.184(2)153-157(2002)
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