Hypoxanthine (Synonyms: 6-Hydroxypurine, NSC 14665, NSC 129419) |
カタログ番号GC10199
One-Click Copy Product Info
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プリン誘導体であるヒポキサンチンは、潜在的なフリーラジカル発生剤であり、低酸素症の指標として使用できます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68-94-0
Sample solution is provided at 25 µL, 10mM.
Hypoxanthine is a metabolite of the purine catabolism pathway[1]. Hypoxanthine can be used as rapid and sensitive bio-marker for acute cardiac ischemia at an early stage[2]. Hypoxanthine acts as a substrate for xanthine oxidase and is associated with ROS (reactive oxygen species), which contribute to vascular dysfunction and atherosclerosis[1].
Hypoxanthine (1.5, 2mM; 2h) induced ROS generation and increased cholesterol levels in HepG2 cells[1].
Hypoxanthine (200mg/kg; 8weeks; p.o.) significantly increased the cholesterol ester levels in Apoe KO mice[1]. Hypoxanthine (135mg/kg; 3d; i.v.) consistently increased the brain infarct volume in C57BL/6J mice underwent middle cerebral artery occlusion surgery[3].
References:
[1] yu H M, Kim Y J, Oh E J, et al. Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E-deficient mice and cells [J]. Journal of cellular and molecular medicine, 2016, 20(11): 2160-2172.
[2] Farthing D E, Farthing C A, Xi L. Inosine and hypoxanthine as novel biomarkers for cardiac ischemia: from bench to point-of-care [J]. Experimental biology and medicine, 2015, 240(6): 821-831.
[3] Ye J, Bi X, Deng S, et al. Hypoxanthine is a metabolic biomarker for inducing GSDME-dependent pyroptosis of endothelial cells during ischemic stroke [J]. Theranostics, 2024, 14(15): 6071-6087.
| Cell experiment [1]: | |
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Cell lines |
Human HepG2 hepatic carcinoma cells |
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Preparation Method |
HepG2 cells were seeded at a density of 1×104 cells/well in a black 96-well plate. Cells were incubated with a solution of 50μM DCFH-DA (2’,7’-dichlorofluorescein diacetate) in serum-free medium for 30min. This was followed by washing the cells twice with PBS. The cells were then incubated with Hypoxanthine for 2h. Intracellular ROS levels were determined using a fluorescence microscope. |
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Reaction Conditions |
1.5, 2mM; 2h |
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Applications |
Hypoxanthine induced ROS generation in HepG2 cells. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6J mice |
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Preparation Method |
Adult C57BL/6J mice were received middle cerebral artery occlusion (MCAO) surgery. Intravenous injection of Hypoxanthine (135mg/kg) was carried out from one day before MCAO to one day after MCAO. Infarct volume assessment by triphenyl tetrazolium chloride (TTC) staining. |
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Dosage form |
135mg/kg; 3d; i.v. |
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Applications |
Hypoxanthine consistently increased the brain infarct volume. |
References: [1] Ryu H M, Kim Y J, Oh E J, et al. Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E-deficient mice and cells [J]. Journal of cellular and molecular medicine, 2016, 20(11): 2160-2172. [2] Ye J, Bi X, Deng S, et al. Hypoxanthine is a metabolic biomarker for inducing GSDME-dependent pyroptosis of endothelial cells during ischemic stroke [J]. Theranostics, 2024, 14(15): 6071-6087. | |
| Cas No. | 68-94-0 | SDF | |
| 同義語 | 6-Hydroxypurine, NSC 14665, NSC 129419 | ||
| Canonical SMILES | O=C1N=CNC2=C1NC=N2 | ||
| Formula | C5H4N4O | M.Wt | 136.11 |
| 溶解度 | ≥ 1.36mg/mL in DMSO with ultrasonic | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 7.347 mL | 36.735 mL | 73.47 mL |
| 5 mM | 1.4694 mL | 7.347 mL | 14.694 mL |
| 10 mM | 734.7 μL | 3.6735 mL | 7.347 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)