mCherry mRNA with N1-Me-pUTP |
| カタログ番号GM10010 |
mCherry mRNAは、分子生物学研究において一般的に使用される蛍光報告遺伝子であり、Cap 1キャップ構造とポリ(A)テールを持ち、N1-Me-pUTP修飾が含まれています。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
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Red fluorescent protein mCherry is a fluorescent reporter gene commonly used in molecular biology research. mCherry mRNA can directly express proteins in the cytoplasm without relying on a promoter. The protein expression speed is faster than transfected DNA. The protein expression level is directly related to the transfection amount of mRNA, and there is no risk of gene integration. After mCherry mRNA is transfected into cells, it can express the strong and bright red fluorescent protein mCherry, with excitation/emission wavelengths of 587 nm/610 nm respectively. mCherry mRNA can be used as a standard to test the transfection efficiency of different transfection reagents, and can also be used as a control to study the transfection and expression of fluorescent proteins in mammalian cells.
By simulating the mRNA processing process in eukaryotes, the product has a Cap 1 cap structure at the 5' end and a poly(A) tail at the 3' end, which increases the stability and translation efficiency of the mRNA[1].
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122
| mRNA Length | 996 nucleotides | ||
| Concentration | 1mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | Please dissolve it on ice and be careful to prevent RNase contamination and degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For first use, gently centrifuge and divide into portions for individual use. Use RNase-free reagents and consumables, use appropriate RNase-free techniques, and do not add to the serum-containing medium until mixed with the transfection reagent. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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