Rhodamine 800 |
カタログ番号GC30591 |
ローダミン800は近赤外蛍光色素です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 137993-41-0
Sample solution is provided at 25 µL, 10mM.
Rhodamine 800 is a cell-permeable cationic red fluorescent probe that specifically identifies mitochondrial membrane potential. The maximum excitation/emission light is 682/704nm. Rhodamine dyes can selectively label mitochondria and measure mitochondrial membrane potential, and are often used to evaluate mitochondrial function through live-cell fluorescence microscopy or flow cytometry. Dyes incorporated into cells are preferentially retained in mitochondria, producing bright fluorescence with fluorescence intensity proportional to mitochondrial membrane potential. Loss of mitochondrial integrity or excessive opening of the mitochondrial permeability transition pore can cause the probe to leak out of the mitochondria, resulting in reduced fluorescence[1]. Rhodamine dye has low toxicity to cells at a certain concentration, so it is often used to detect mitochondria in animal cells, plant cells, and microorganisms[2].
References:
[1]. Cristiano Ferlini, Giovanni Scambia. Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange. 2007;2(12):3111-4. doi: 10.1038/nprot.2007.397.
[2]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
Average Rating: 5
(Based on Reviews and 11 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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