TMRM |
カタログ番号GC30553 |
TMRM は、細胞透過性カチオン性親油性赤色蛍光色素です (Λex=530 nm、Λem=592 nm)。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 115532-49-5
Sample solution is provided at 25 µL, 10mM.
TMRM is a cell-permeable cationic red fluorescent probe that specifically recognizes mitochondrial membrane potential with a maximum excitation/emission light of 552/575nm[1]. TMRM can selectively label mitochondria and measure mitochondrial membrane potential, and is often used to evaluate mitochondrial function through live cell fluorescence microscopy or flow cytometry. TMRM incorporated into cells is preferentially retained in mitochondria, producing bright fluorescence, and the fluorescence intensity is proportional to the mitochondrial membrane potential. Loss of mitochondrial integrity or excessive opening of the mitochondrial permeability transition pore can cause the probe to leak out of the mitochondria, resulting in reduced fluorescence[2]. Rhodamine dye has low toxicity to cells at a certain concentration, so it is often used to detect mitochondria in animal cells, plant cells, and microorganisms[3].
References:
[1]. Michael B Schultz,et. Molecular and Cellular Characterization of SIRT1 Allosteric Activators. 2019:1983:133-149. doi: 10.1007/978-1-4939-9434-2_8.
[2]. Cristiano Ferlini, Giovanni Scambia. Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange. 2007;2(12):3111-4. doi: 10.1038/nprot.2007.397.
[3]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
Average Rating: 5
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