TPh A |
カタログ番号GC61815
One-Click Copy Product Info
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TPh A (トリフェニル化合物 A) は、核タンパク質ピリンの強力な阻害剤であり、0.6 uM の Ki でピリンに特異的に結合します。 TPh A は、bcl3-ピリン複合体の形成を妨害します。 TPh A は、細胞内のピリンを調節するための新しい小分子ツールとして使用できます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21306-65-0
Sample solution is provided at 25 µL, 10mM.
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TPh A (Triphenyl Compound A) is a potent inhibitor of the nuclear protein pirin and binds specifically to pirin with a Ki of 0.6 uM. TPh A disrupts the formation of the bcl3-pirin complex. TPh A can be used as a novel small molecule tool to regulate pirin in cells[1].
The Pirin is a nuclear protein with the nuclear factor I/CCAAT box transcription factor (NFI/CTF). Pirin proteins are highly conserved between mammals, plants, fungi, and prokaryotic organisms and are considered to belong to the cupin superfamily.TPh A (20 µM; 5 hours) reduces the amount of pirin-bound Bcl3 and inhibits the interaction between pirin and Bcl3 in HEK293T cells in a glutathione S-transferase (GST) pulldown assay (HEK293T cells are transfected with vectors that encoded bcl3-Myc and pirin-His6 for 43 h of transfection)[1].TPh A (0-100 µM; 48 hours) does not exert any potent cytotoxic activity against many human cancer cell lines, it against MCF7, MDA-MB231, HeLa, DU145, HepG2, A549, HT1080, WM266-4, and SK-MEL-28 cells with IC50 values >50 µM, and exhibits IC50 values of 27 µM, 20 µM and 26 µM for PC3 HL60 and HT29 cells, respectively[1].TPhA (0-50 µM; 48 hours) inhibits cell migration in WM266-4 cells, SK-MEL-28 cell in a concentration-dependent manner[1].
References:
[1]. Isao Miyazaki, et al. A Small-Molecule Inhibitor Shows That Pirin Regulates Migration of Melanoma Cells. Nat Chem Biol. 2010 Sep;6(9):667-73.
| Cas No. | 21306-65-0 | SDF | |
| Canonical SMILES | O=[S](/N=[S](C(C=C1)=CC=C1OCC2=CC=CC=C2)\C)(C(C=C3)=CC=C3C)=O | ||
| Formula | C21H21NO3S2 | M.Wt | 399.53 |
| 溶解度 | DMSO : 250 mg/mL (625.74 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5029 mL | 12.5147 mL | 25.0294 mL |
| 5 mM | 500.6 μL | 2.5029 mL | 5.0059 mL |
| 10 mM | 250.3 μL | 1.2515 mL | 2.5029 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)