UDP-α-D-Glucose (sodium salt) (Synonyms: NSC 20269, UDPG, UDP-Glucose, Uridine-5'-diphosphoglucose) |
カタログ番号GC45111
One-Click Copy Product Info
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UDP-α-D-グルコース (ナトリウム塩) (UDP-D-グルコース二ナトリウム塩) は、動物組織および一部の微生物におけるグルコース含有オリゴ糖、多糖類、糖タンパク質、および糖脂質の前駆体です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 28053-08-9
Sample solution is provided at 25 µL, 10mM.
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UDP-α-D-Glucose (sodium salt) is an endogenous nucleotide sugar composed of uridine diphosphate and α-D-glucose. UDP-α-D-Glucose has a well established biochemical role as a glycosyl donor in the enzymatic biosynthesis of carbohydrates. UDP-α-D-Glucose is also an agonist of the G protein-coupled receptor KIAA0001 (P2Y14), which is expressed in neutrophils and is involved in activation of inflammatory signaling[1][2].
In vitro, UDP-α-D-glucose (100μM; 4h) increased ERK1/2 phosphorylation and upregulated IL-8 and CCL2 mRNA expression in MDCK-C11 cells[3]. UDP-α-D-glucose (30μM; 2h) enhanced the random migration of eosinophils[4].
In vivo, UDP-α-D-Glucose (200mg/kg/d; s.c.; 6d) promoted the mobilization of hematopoietic stem and progenitor cells (HSPCs), as evidenced by a significant increase in circulating Colony-Forming Units in Culture (CFU-Cs) in the blood of both B6 and BALB/c mice, including CFU-Granulocyte, Macrophage (CFU-GM), Burst-Forming Unit-Erythroid (BFU-E), and CFU-Granulocyte, Erythroid, Macrophage, Megakaryocyte (CFU-GEMM)[5]. UDP-α-D-Glucose (500-2000mg/kg; i.p.; once) dose-dependently reduced gastric emptying in the conscious rat[6].
References:
[1] Chambers JK, Macdonald LE, Sarau HM, et al. A G protein-coupled receptor for UDP-glucose. J Biol Chem. 2000;275(15):10767-10771.
[2] Li K, Zhou P, Li J, et al. Upregulation of P2Y14 receptor in neutrophils promotes inflammation after myocardial ischemia/reperfusion injury. Life Sci. 2023;326:121805.
[3] Azroyan A, Cortez-Retamozo V, Bouley R, et al. Renal intercalated cells sense and mediate inflammation via the P2Y14 receptor. PLoS One. 2015;10(3):e0121419.
[4] Karcz TP, Whitehead GS, Nakano K, et al. UDP-glucose and P2Y14 receptor amplify allergen-induced airway eosinophilia. J Clin Invest. 2021;131(7):e140709.
[5] Kook S, Cho J, Lee SB, Lee BC. The nucleotide sugar UDP-glucose mobilizes long-term repopulating primitive hematopoietic cells. J Clin Invest. 2013;123(8):3420-3435.
[6] Bassil AK, Bourdu S, Townson KA, et al. UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms. Am J Physiol Gastrointest Liver Physiol. 2009;296(4):G923-G930.
| Cell experiment [1]: | |
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Cell lines |
Human neutrophils |
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Preparation Method |
Neutrophils (2×106/ml) were incubated in HBSS in the presence of 100μM UDP-α-D-Glucose or 100μM ATP for 60min. At the times indicated, cells were centrifuged and nucleotides present in the supernatant were separated by HPLC (Waters) via a Nova-Pack C18 column. Absorbance at λ=260nm and radioactivity were quantified on-line as previously described. |
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Reaction Conditions |
100μM; 60min |
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Applications |
Incubation of neutrophils with 100μM UDP-α-D-Glucose for 60min resulted in negligible metabolism of the nucleotide-sugar, i.e., <1% of the initial UDP-α-D-Glucose was recovered as UMP. In contrast, neutrophils rapidly hydrolyzed 100μM ATP, displaying an apparent hydrolysis rate of Vhyd=7.3nmol/min×106 cells. |
| Animal experiment [2]: | |
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Animal models |
Male CD rats |
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Preparation Method |
Male CD rats were acclimatized to being singly housed in cages for at least 3 days before experimental use. The animals were fasted overnight before the experiment but were allowed free access to drinking water at all times. On the day of the experiment, UDP-α-D-Glucose dissolved in 0.9% wt/vol sodium chloride was administered by intraperitoneal (i.p.) injection at 500, 1000, and 2000mg/kg in a total volume of 2ml/kg. Control animals received 0.9% wt/vol i.p. sodium chloride (saline), and, 10min later, 3ml of the nutrient meal was administered orally. 60min later, animals were culled, stomach contents were weighed, and the percentage of gastric emptying was calculated. |
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Dosage form |
500, 1000, and 2000mg/kg; i.p.; once |
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Applications |
UDP-α-D-Glucose dose-dependently reduced gastric emptying in the conscious rat. |
References: [1] Sesma JI, Kreda SM, Steinckwich-Besancon N, et al. The UDP-sugar-sensing P2Y(14) receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils. Am J Physiol Cell Physiol. 2012;303(5):C490-C498. [2] Bassil AK, Bourdu S, Townson KA, et al. UDP-glucose modulates gastric function through P2Y14 receptor-dependent and -independent mechanisms. Am J Physiol Gastrointest Liver Physiol. 2009;296(4):G923-G930. | |
| Cas No. | 28053-08-9 | SDF | |
| 同義語 | NSC 20269, UDPG, UDP-Glucose, Uridine-5'-diphosphoglucose | ||
| Canonical SMILES | O[C@@H]1[C@@H](COP(OP(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)([O-])=O)([O-])=O)O[C@@H](N3C=CC(NC3=O)=O)[C@@H]1O.[Na+].[Na+] | ||
| Formula | C15H22N2O17P2•2Na | M.Wt | 610.3 |
| 溶解度 | Water : 125 mg/mL (204.83 mM) | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6385 mL | 8.1927 mL | 16.3854 mL |
| 5 mM | 327.7 μL | 1.6385 mL | 3.2771 mL |
| 10 mM | 163.9 μL | 819.3 μL | 1.6385 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)