(±)12(13)-DiHOME-d4 (Synonyms: Isoleukotoxin diold4) |
| Catalog No.GC46256 |
A neuropeptide with diverse biological activities
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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(±)12(13)-DiHOME-d4 is intended for use as an internal standard for the quantification of (±)12(13)-DiHOME by GC- or LC-MS. (±)12(13)-DiHOME is the diol form of (±)12(13)-EpOME, a cytochrome P450-derived epoxide of linoleic acid also known as isoleukotoxin.1 It is formed from 12(13)-EpOME by soluble epoxide hydrolase (sEH) in neutrophils.2 12(13)-DiHOME is toxic to Sf21 cells expressing sEH and to lacZ-expressing control cells, unlike isoleukotoxin, which is only toxic to cells containing sEH.1,2 Levels of 12(13)-DiHOME are increased in rat spinal cord following burn injury, and it enhances cold tolerance, increases fatty acid uptake into brown adipocytes, and decreases serum triglyceride levels in mice.3,4 Levels are also elevated in bronchoalveolar lavage fluid (BALF) in humans following exposure to biodiesel exhaust and in exhaled breath condensate in patients with asthma following allergen exposure.5,6 Plasma levels of 12(13)-DiHOME are increased immediately following moderate-intensity exercise in mice and humans, an effect that can be prevented by brown adipose tissue removal in the mouse.7
1.Greene, J.F., Williamson, K.C., Newman, J.W., et al.Metabolism of monoepoxides of methyl linoleate: Bioactivation and detoxificationArch. Biochem. Biophys.376(2)420-432(2000) 2.Moghaddam, M.F., Grant, D.F., Cheek, J.M., et al.Bioactivation of leukotoxins to their toxic diols by epoxide hydrolaseNat. Med.3(5)562-566(1997) 3.Green, D.P., Ruparel, S., Gao, X., et al.Central activation of TRPV1 and TRPA1 by novel endogenous agonists contributes to mechanical allodynia and thermal hyperalgesia after burn injuryMol. Pain12(2016) 4.Lynes, M.D., Leiria, L.O., Lundh, M., et al.The cold-induced lipokine 12,13-diHOME promotes fatty acid transport into brown adipose tissueNat. Med.23(5)631-637(2017) 5.Gouveia-Figueira, S., Karimpour, M., Bosson, J.A., et al.Mass spectrometry profiling of oxylipins, endocannabinoids, and N-acylethanolamines in human lung lavage fluids reveals responsiveness of prostaglandin E2 and associated lipid metabolites to biodiesel exhaust exposureAnal. Bioanal. Chem.409(11)2967-2980(2017) 6.Nording, M.L., Yang, J., Hegedus, C.M., et al.Endogenous levels of five fatty acid metabolites in exhaled breath condensate to monitor asthma by high-performance liquid chromatography: Electrospray tandem mass spectrometryIEEE Sens. J.10(1)123-130(2010) 7.Stanford, K.I., Lynes, M.D., Takahashi, H., et al.12,13-diHOME: An exercise-induced lipokine that increases skeletal muscle fatty acid uptakeCell Metab.27(5)1111-1120(2018)
| Cas No. | N/A | SDF | |
| Synonyms | Isoleukotoxin diold4 | ||
| Canonical SMILES | CCCCC[C@](O)([2H])[C@](O)([2H])C/C([2H])=C([2H])\CCCCCCCC(O)=O | ||
| Formula | C18H30D4O4 | M.Wt | 318.5 |
| Solubility | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,PBS (pH 7.2): 10 μg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.1397 mL | 15.6986 mL | 31.3972 mL |
| 5 mM | 0.6279 mL | 3.1397 mL | 6.2794 mL |
| 10 mM | 0.314 mL | 1.5699 mL | 3.1397 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)
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