α-(difluoromethyl)-DL-Arginine (Synonyms: DFMA, RMI 71897) |
| Catalog No.GC45194 |
Bacteria synthesize the cellular growth factor putrescine through a number of pathways.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 69955-43-7
Sample solution is provided at 25 µL, 10mM.
Bacteria synthesize the cellular growth factor putrescine through a number of pathways. One pathway involves the decarboxylation of arginine by arginine decarboxylase to produce agmatine, which is then degraded to putrescine. While important for various cellular processes (e.g., cell division, differentiation, environmental stress responses) in all living organisms, including plants, high levels of this polyamine can be toxic.[1] α-(difluoromethyl)-DL-Arginine (DFMA) is an enzyme-activated, irreversible inhibitor of the arginine decarboxylases of E. coli (Ki = 800 µM), P. aeruginosa, and K. pneumoniae.[2] At 0.01 mM, it has been shown to prevent the osmotic stress-induced increase in arginine decarboxylase activity and putrescine synthesis in oat leaf cells.[3],[4] DFMA can also reduce the growth of T. cruzi in mammalian host cells at a minimal concentration of 10 mM and prevent the growth of C. parvum in a T cell receptor alpha-deficient mouse model when combined with various polyamine analogs.[5],[6]
Reference:
[1]. Gill, S.S., and Tuteja, N. Polyamines and abiotic stress tolerance in plants. Plant Signal. Behav. 5(1), 26-33 (2010).
[2]. Kallio, A., McCann, P.P., and Bey, P. DL-α-(Difluoromethyl)arginine: A potent enzyme-activated irreversible inhibitor of bacterial decarboxylases. Biochemistry 20(11), 3163-3168 (1981).
[3]. Tiburcio, A.F., Kaur-Sawhney, R., and Galston, A.W. Polyamine metabolism and osmotic stress. II. Improvement of oat protoplasts by an inhibitor of arginine decarboxylase. Plant Physiology 82, 375-378 (1986).
[4]. Flores, H.E., and Galston, A.W. Polyamines and plant stress: Activation of putrescine biosynthesis by osmotic shock. Science 217(4566), 1259-1261 (1982).
[5]. Kierszenbaum, F., Wirth, J.J., McCann, P.P., et al. Arginine decarboxylase inhibitors reduce the capacity of Trypanosoma cruzi to infect and multiply in mammalian host cells. Proceedings of the National Academy of Sciences of the United States of America 84(12), 4278-4282 (1987).
[6]. Yarlett, N., Waters, W.R., Harp, J.A., et al. Activities of DL-α-difluoromethylarginine and polyamine analogues against Cryptosporidium parvum infection in a T-cell receptor α-deficient mouse model. Antimicrobial Agents and Chemotherapy 51(4), 1234-1239 (2007).
| Cas No. | 69955-43-7 | SDF | |
| Synonyms | DFMA, RMI 71897 | ||
| Chemical Name | 2-(difluoromethyl)arginine | ||
| Canonical SMILES | FC(F)C(C(O)=O)(N)CCCNC(N)=N | ||
| Formula | C7H14F2N4O2 | M.Wt | 224.2 |
| Solubility | PBS (pH 7.2): 5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.4603 mL | 22.3015 mL | 44.603 mL |
| 5 mM | 0.8921 mL | 4.4603 mL | 8.9206 mL |
| 10 mM | 0.446 mL | 2.2302 mL | 4.4603 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *