β-D-Glucan |
Catalog No.GC66870
One-Click Copy Product Info
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β-D-글루칸은 천연 비소화성 다당류이며 Dectin-1 및 Toll 유사 수용체와 같은 인식 수용체에 의해 선택적으로 인식될 수 있을 뿐만 아니라 쥐 또는 인간 대식세포에 의해 쉽게 내재화될 수 있는 높은 생체 적합성입니다. 821d96072c2d58d8970e76f526b0f6b8목표 배송에 대한 속성입니다. 821d96072c2d58d8970e76f526b0f6b8β-d-글루칸은 프로바이오틱스의 장용 전달 수단입니다.821d96072c2d58d8970e76f526b0f6b8
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9041-22-9
Sample solution is provided at 25 µL, 10mM.
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β-D-Glucan is an element of the cyst wall of P. jirovecii (and of the cell wall of most other fungi), that can trigger various host inflammatory responses [1]. Under the condition of constant temperature and stirring at 90℃, β-D-Glucan can be used to prepare stable nanoparticles for killing P. aphanidermatum [2]. β-D-Glucan is widely used as a marker for diagnosing Pneumocystis carinii pneumonia and as a prognostic biomarker for invasive candidiasis (IC) [3-4].
In vitro, β-D-glucan treatment for 72h inhibited tamoxifen/endocrine-resistant LCC9 and LY2 cell proliferation with IC50 values of 4.6μg/ml and 24.2μg/ml, respectively [5].
In vivo, β-D-glucan treatment via oral administration at a dose of 350mg/kg once daily for 14 consecutive days significantly increased the survival rate of mice from 6Gy X-rays irradiation[6]. Oral administration of 200mg/kg of β-D-glucan daily for 16 days significantly inhibited the tumor growth in S180 tumor-bearing mice and induced apoptosis of tumor cells[7].
References:
[1] Karageorgopoulos D E, Qu J M, Korbila I P, et al. Accuracy of β-D-glucan for the diagnosis of Pneumocystis jirovecii pneumonia: a meta-analysis[J]. Clinical Microbiology and Infection, 2013, 19(1): 39-49.
[2] Anusuya S, Sathiyabama M. Preparation of β-d-glucan nanoparticles and its antifungal activity[J]. International Journal of Biological Macromolecules, 2014, 70: 440-443.
[3] Theel E S, Doern C D. Point-counterpoint: β-d-glucan testing is important for diagnosis of invasive fungal infections[J]. Journal of clinical microbiology, 2013, 51(11): 3478-3483.
[4] Jaijakul S, Vazquez J A, Swanson R N, et al. (1, 3)-β-D-glucan as a prognostic marker of treatment response in invasive candidiasis[J]. Clinical infectious diseases, 2012, 55(4): 521-526.
[5] Jafaar Z M T, Litchfield L M, Ivanova M M, et al. β-D-glucan inhibits endocrine-resistant breast cancer cell proliferation and alters gene expression[J]. International Journal of Oncology, 2014, 44(4): 1365-1375.
[6] Liu F, Wang Z, Liu J, et al. Radioprotective effect of orally administered beta-d-glucan derived from Saccharomyces cerevisiae[J]. International journal of biological macromolecules, 2018, 115: 572-579.
[7] Mo L, Chen Y, Li W, et al. Anti-tumor effects of (1→ 3)-β-d-glucan from Saccharomyces cerevisiae in S180 tumor-bearing mice[J]. International journal of biological macromolecules, 2017, 95: 385-392.
| Cell experiment [1]: | |
Cell lines | MCF-7 cells |
Preparation Method | MCF-7 cells were grown in IMEM medium supplemented with 10% fetal bovine serum in 5% CO2 at 37°C. The exponentially growing MCF-7 cells were seeded into 96-well microplates at a density of 5×103 cells/well for 24h. Afterwards, the medium was removed, and cells were treated with different concentrations of β-D-glucan (0, 1, 10, 50, 100, 200, 300, and 400μg/ml). At the end of the incubation period of 72h at 37°C, cell viability was analyzed. |
Reaction Conditions | 0, 1, 10, 50, 100, 200, 300, and 400μg/ml; 72h |
Applications | β-D-glucan treatment significantly reduced the cell viability of MCF-7 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Female SPF BALB/c mice |
Preparation Method | Female C57BL/6J mice (8 weeks old; 20-22g) were maintained under a 12-h light and 12-h dark cycle at a temperature of 24±1°C and humidity of 50%±10%, with free water and diet. After one-week acclimatization, mice were divided into three groups randomly: control (C); irradiated with X-rays (IR); pretreated with β-D-glucan and irradiated with X-rays (BG+IR), orally gavage of β-D-glucan (350mg/kg) once a day for 14 days. Then both of the mice in group IR and group BG+IR were placed into plastic tubes with breathing holes and were total body irradiated (TBI) with sublethal dose (6Gy) X-rays. The number of surviving mice in three groups (group C, group IR and group BG+IR) were monitored daily for 30 days. |
Dosage form | 350mg/kg/day for 14 days; p.o. |
Applications | β-D-glucan treatment increased the survival rate of mice from X-ray irradiation. |
References: | |
| Cas No. | 9041-22-9 | SDF | |
| Formula | M.Wt | ||
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)