>>Signaling Pathways>> PI3K/Akt/mTOR Signaling>> AMPK>>Ampkinone

Ampkinone

Catalog No.GC16534

Ampkinone은 간접 AMP 활성화 단백질 키나제(AMPK) 활성화제입니다.

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Ampkinone Chemical Structure

Cas No.: 1233082-79-5

Size 가격 재고 수량
1mg
US$34.00
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5mg
US$130.00
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10mg
US$214.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Kinase experiment:

Total AMPK activity is measured using a synthetic SAMS peptide substrate and [γ-32P]ATP. Briefly, 500 μg of protein extract is incubated with anti-AMPK-α1 and α2 antibodies for 2 h at 4°C. Protein A/G sepharose and agarose are added, and the mixtures are incubated for 3 h at 4°C. After the samples are washed three times with RIPA buffer, the activity is assessed in AMPK reaction buffer containing 20 mM HEPES-NaOH (pH 7.0), 0.4 mM Dithiothreitol, and 0.01% Brij-35, with or without 300 μM AMP. The immune complexes are added to 23 μL of reaction buffer per assay, and then 7 μL of SAMS substrate peptide (HMRSAMSGLHLVKRR, 100 μM final concentration) and 10 μL of ATP mixture (an aliquot of 1 μCi/μL: 90 μL of 75 mM magnesium chloride, 500 μM unlabeled ATP in 20 mM MOPS, pH7.2, 25 mMβ-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate, and 1 mM Dithiothreitol) are added, and the mixture is incubated at 30°C for 15 min. After 35 μL is spotted onto the center of P81 paper, the paper is washed three times with 0.75% phosphoric acid and once with acetone for 5 min. The samples are read using a scintillation counter[1].

Animal experiment:

Mice[1]C57BL/6J mice are used and housed individually in a room maintained at 25°C on a 12/12 h light/dark schedule. For dietinduced obesity (DIO) mice, 4-week-old male C57BL/6J mice are fed a high-fat diet (HFD, 60% calories from fat) ad libitum for 8 weeks. Ampkinone in polyethylene glycol (PEG400) or vehicle is administered subcutaneously at 10 mg/kg body weight per day for 1 month. Body weight and food intake are measured every 3 days. All animals are given an insulin tolerance test (ITT) and sacrificed at 30 days. Liver weight and fat masses are measured, sectioned, and stained with Oil-red O and H&E.

References:

[1]. Oh S, et al. Antidiabetic and antiobesity effects of Ampkinone (6f), a novel small molecule activator of AMP-activated protein kinase. J Med Chem. 2010 Oct 28;53(20):7405-13.

Background

Ampkinone is an AMPK activator.

Adenosine 5'-monophosphate (AMP) activated protein kinase (AMPK) has considered as an promising target molecule for the treatment of metabolic disorders, such as obesity and type 2 diabetes.

In vitro: Ampkinone was identified as an indirect AMPK activator, which was derived from the small molecule library constructed by diversity-oriented synthesis. Ampkinone was able to stimulate the phosphorylation of AMPK through the indirect activation of AMPK in various cell lines. Moreover, ampkinone-mediated activation of AMPK needed the activity of LKB1 and led to increased glucose uptake in muscle cells [1].

In vivo: Animal study found that ampkinone-treated DIO mice had significantly reduced total body weight and overall fat mass. Histological examination and measurement of lipid parameters showed that ampkinone could effectively improve metabolic abnormalities. These results demonstrated that ampkinone had a potential as a new class of therapeutic agent for antidiabetic and antiobesity treatment through the indirect stimulation of AMPK [1].

Clinical trial: So far, no clinical study has been conducted.

Reference:
[1] Oh, S. ,Kim, S.J.,Hwang, J.H., et al. Antidiabetic and antiobesity effects of ampkinone (6f), a novel small molecule activator of AMP-activated protein kinase. Journal of Medicinal Chemistry 53, 7405-7413 (2010).

Chemical Properties

Cas No. 1233082-79-5 SDF
Chemical Name 2-(4-benzoylphenyl)-6-hydroxy-7-methoxy-4,4-dimethyl-[1]benzopyrano[3,4-e]isoindole-1,3(2H,4H)-dione
Canonical SMILES O=C1N(C2=CC=C(C(C3=CC=CC=C3)=O)C=C2)C(C4=C1C=CC5=C4C(C)(C)OC6=C(O)C(OC)=CC=C65)=O
Formula C31H23NO6 M.Wt 505.5
Solubility ≤2mg/ml in DMSO;10mg/ml in dimethyl formamide Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.9782 mL 9.8912 mL 19.7824 mL
5 mM 0.3956 mL 1.9782 mL 3.9565 mL
10 mM 0.1978 mL 0.9891 mL 1.9782 mL
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리뷰

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Average Rating: 5 ★★★★★ (Based on Reviews and 33 reference(s) in Google Scholar.)

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