Arachidonic acid (Immunocytophyt) (Synonyms: Single-Use AA (peroxide free)) |
Catalog No.GC31725
One-Click Copy Product Info
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Arachidonic acid (Immunocytophyt)은 동물과 인간의 지방에 있고 간, 뇌 및 선체 기관에도 발견되는 필수 불포화 지방산이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 506-32-1
Sample solution is provided at 25 µL, 10mM.
Arachidonic acid (Immunocytophyt)은 동물과 인간의 지방에 있고 간, 뇌 및 선체 기관에도 발견되는 필수 불포화 지방산이다. 동물의 인산화질 중 하나이다. 프로스타글란딘과 룩트리엔의 생물합성의 전신이다. Arachidonic acid은 세포 신호 전달과 인플렘마 발생의 중요한 요소이다[1-3].
Arachidonic acid (0-40 μM; 3분)은 혈관 세포에서 단백질 틸로신 인산화을 자극한다[4]. Arachidonic acid (3.3μM; 24시간)은 EB 형성을 지원하고 Valproic acid (VA) 유도의 태아 독성을 EB로부터 보호한다[5].
Arachidonic acid (1-4mg/kg; 구강 투여; 매일 1회, 3주간) 치료는 비만 쥐에서 pro-염증 표지자와 COX 경로의 현저한 다운-규레이션을 초래했고 12/15-LOX의 업-규레이션이 관찰되었다[6]. Arachidonic acid + 식사로는 Tg2576 쥐에서 엠스타티드 전구 단백질(APP) 처리의 변화를 통해 아밀로이드 β-단백(Aβ) 침전을 예방할 수 있다[7].
References:
[1]. Tallima H, El Ridi R. Arachidonic acid: Physiological roles and potential health benefits - A review. J Adv Res. 2017 Nov 24;11:33-41. doi: 10.1016/j.jare.2017.11.004. PMID: 30034874; PMCID: PMC6052655.
[2]. Wang B, Wu L,et,al. Metabolism pathways of arachidonic acids: mechanisms and potential therapeutic targets. Signal Transduct Target Ther. 2021 Feb 26;6(1):94. doi: 10.1038/s41392-020-00443-w. PMID: 33637672; PMCID: PMC7910446.
[3]. Turolo S, Edefonti A, et,al. Role of Arachidonic Acid and Its Metabolites in the Biological and Clinical Manifestations of Idiopathic Nephrotic Syndrome. Int J Mol Sci. 2021 May 21;22(11):5452. doi: 10.3390/ijms22115452. PMID: 34064238; PMCID: PMC8196840.
[4]. Buckley BJ, Whorton AR. Arachidonic acid stimulates protein tyrosine phosphorylation in vascular cells. Am J Physiol. 1995 Dec;269(6 Pt 1):C1489-95. doi: 10.1152/ajpcell.1995.269.6.C1489. PMID: 8572178.
[5]. Nihad M, Sen U, et,al. Arachidonic acid modulates the cellular energetics of human pluripotent stem cells and protects the embryoid bodies from embryotoxicity effects in vitro. Reprod Toxicol. 2023 Sep;120:108438. doi: 10.1016/j.reprotox.2023.108438. Epub 2023 Jul 16. PMID: 37454977.
[6]. Roy S, Ripon MAR, et,al. Arachidonic acid supplementation attenuates adipocyte inflammation but not adiposity in high fat diet induced obese mice. Biochem Biophys Res Commun. 2022 Jun 11;608:90-95. doi: 10.1016/j.bbrc.2022.03.089. Epub 2022 Mar 29. PMID: 35397428.
[7]. Hosono T, Nishitsuji K, et,al. Arachidonic acid diet attenuates brain Aβ deposition in Tg2576 mice. Brain Res. 2015 Jul 10;1613:92-9. doi: 10.1016/j.brainres.2015.04.005. Epub 2015 Apr 13. PMID: 25881896.
| 세포 실험 [1]: | |
세포 라인 | 배아체 (EBs) |
제조 방법 | 배아체(EBs)는 24웰 판에 심어져 다양한 농도의 Arachidonic acid만 발프로산(VA)만 그리고 VA와 3.3 µM Arachidonic acid의 조합으로 처리되었습니다. |
반응 조건 | 3.3µM;24시간 |
응용 분야 | Arachidonic acid은 EB 형성을 지원하고Valproic acid (VA) 유도의 태아 독성을 EB로부터 보호합니다. |
| 동물 실험 [2]: | |
동물 모형 | Swiss Albino 수컷 쥐 (고지 식사를 통한 비만 유도) |
제조 방법 | 쥐는 세 그룹으로 나뉘었습니다. 두 쥐 그룹은 1 mg/kg의 체중을 위한 저농도 Arachidonic acid (LAA)과 4 mg/kg의 체중을 위한 중농도 Arachidonic acid (MAA)을 각각 일일 한 번에 구강 투여로 주입받았고 동시에 고지 식사 (HFD)를 했습니다. 나머지 비만 쥐 그룹은 HFD를 계속했습니다. |
제형 | 1-4mg/kg; i.g; 3주 동안 매일 한번 |
응용 분야 | Arachidonic acid 치료는 비만 쥐에서 pro-염증 표지자와 COX 경로의 현저한 다운-규레이션을 초래했습니다. |
참고문헌: [1]. Nihad M, Sen U, et,al. Arachidonic acid modulates the cellular energetics of human pluripotent stem cells and protects the embryoid bodies from embryotoxicity effects in vitro. Reprod Toxicol. 2023 Sep;120:108438. doi: 10.1016/j.reprotox.2023.108438. Epub 2023 Jul 16. PMID: 37454977.[2]. Roy S, Ripon MAR, et,al. Arachidonic acid supplementation attenuates adipocyte inflammation but not adiposity in high fat diet induced obese mice. Biochem Biophys Res Commun. 2022 Jun 11;608:90-95. doi: 10.1016/j.bbrc.2022.03.089. Epub 2022 Mar 29. PMID: 35397428. | |
| Cas No. | 506-32-1 | SDF | |
| Synonyms | Single-Use AA (peroxide free) | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O | ||
| Formula | C20H32O2 | M.Wt | 304.47 |
| Solubility | DMSO : ≥ 50 mg/mL (164.22 mM);Ethanol : ≥ 50 mg/mL (164.22 mM);Water : < 0.1 mg/mL (insoluble) | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2844 mL | 16.422 mL | 32.844 mL |
| 5 mM | 656.9 μL | 3.2844 mL | 6.5688 mL |
| 10 mM | 328.4 μL | 1.6422 mL | 3.2844 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)