Arachidonic acid (Immunocytophyt) (Synonyms: Single-Use AA (peroxide free)) |
| Catalog No.GC31725 |
아라키돈산(Immunocytophyt)은 필수 지방산이며 생체막의 주요 성분입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 506-32-1
Sample solution is provided at 25 µL, 10mM.
Product has been cited by 1 publications
Product Documents
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Protocol
| Cell experiment [1]: | |
|
Cell lines |
Embryoid bodies (EBs) |
|
Preparation Method |
Embryoid bodies (EBs) were seeded in 24-well plates and treated with various concentrations of Arachidonic acid alone, Valproic acid (VA) alone and VA in combination with 3.3 µM Arachidonic acid. |
|
Reaction Conditions |
3.3µM;24h |
|
Applications |
Arachidonic acid supports EB formation and protects EBs from Valproic acid (VA)-induced embryotoxicity. |
| Animal experiment [2]: | |
|
Animal models |
Swiss Albino male mice (feeding high-fat diets to induce obesity) |
|
Preparation Method |
Mice groups were divided into three groups. Two groups of mice received two different doses of AA, which were 1 mg/kg of body weight named Low Arachidonic Acid (LAA) and 4 mg/kg of body weight named Moderate Arachidonic Acid (MAA) once daily through oral gavage, along with HFD(high-fat diets). The remaining obese group of mice was continued with HFD. |
|
Dosage form |
1-4mg/kg; i.g; once daily for 3weeks |
|
Applications |
Arachidonic acid treatment has resulted in a significant down-regulation of pro-inflammatory markers as well as the COX pathway in obesity mice. |
|
References: [1]. Nihad M, Sen U, et,al. Arachidonic acid modulates the cellular energetics of human pluripotent stem cells and protects the embryoid bodies from embryotoxicity effects in vitro. Reprod Toxicol. 2023 Sep;120:108438. doi: 10.1016/j.reprotox.2023.108438. Epub 2023 Jul 16. PMID: 37454977. |
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아라키돈산은 필수 지방산이며 생체막의 주요 성분입니다.
아라키돈산(ARA)은 프로스타글란딘 E2(PGE2)와 같은 다양한 지질 매개체로 전환되며, 이는 류머티스 관절염(RA) 발병에 연관됩니다[1].
References:
[1]. Tateishi N, et al. Dietary supplementation with arachidonic acid increases arachidonic acid content in paw, but does not affect arthritis severity or prostaglandin E2 content in rat adjuvant-induced arthritis model. Lipids Health Dis. 2015 Jan 16;14:3.
| Cas No. | 506-32-1 | SDF | |
| Synonyms | Single-Use AA (peroxide free) | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O | ||
| Formula | C20H32O2 | M.Wt | 304.47 |
| Solubility | DMSO : ≥ 50 mg/mL (164.22 mM);Ethanol : ≥ 50 mg/mL (164.22 mM);Water : < 0.1 mg/mL (insoluble) | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Complete Stock Solution Preparation Table
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2844 mL | 16.422 mL | 32.844 mL |
| 5 mM | 0.6569 mL | 3.2844 mL | 6.5688 mL |
| 10 mM | 0.3284 mL | 1.6422 mL | 3.2844 mL |
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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리뷰
Average Rating: 5 ★★★★★ (Based on Reviews and 2 reference(s) in Google Scholar.)
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