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BODIPY 505/515 Sale

Catalog No.GC42960

BODIPY 505/515은 지방친화성 형광 염료로, 지질 드롭렛 라벨링에 널리 사용되며 (Ex/Em: 505/515 nm) 형광을 방출합니다.

Products are for research use only. Not for human use. We do not sell to patients.

BODIPY 505/515 Chemical Structure

Cas No.: 21658-70-8

Size 가격 재고 수량
100mg
US$74.00
재고 있음
250mg
US$166.00
재고 있음
500mg
US$315.00
재고 있음

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 4 publications

Product Documents

Quality Control & SDS

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Protocol

Procedure for BODIPY 505/515 staining of Aurantiochytrium sp. [1]

A 5 mM BODIPY 505/515 stock was prepared by dissolving in dimethyl sulfoxide and stored in the dark.

For efficient staining of the intracellular lipid bodies in Aurantiochytrium sp., an aliquot of 0.2% DMSO (vol/vol) was added to the microalgal suspension. 2 μL of BODIPY 505/515 solution was added into the l ml of algal-DMSO suspension and gently shaken for 1 min. Samples were then incubated in darkness for 5–10 min at room temperature. Then the samples were directly used for FACS and microscopic analyses

Procedure for BODIPY 505/515 staining of HepG2 and MCF7 cells. [2]

HepG2 and MCF7 cells were washed once with PBS and fixed in 4% paraformaldehyde for 15 min. Cells were washed three times with PBS and incubated with 0.2 μM BODIPY 505/515 for 30 min. The cells were then counterstained with 1.5 μg/mL DAPI for 5 min. Cells were examined and photographed using the fluorescence microscopy.

This protocol only provides a guideline, and should be modified according to your specific needs

References:

[1]. Velmurugan N, Sathishkumar Y, Yim S S, et al. Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101[J]. Bioresource technology, 2014, 161: 149-154.

[2]. Jhu J W, Yan J B, Lin Z H, et al. SREBP1-Induced Glutamine Synthetase Triggers a Feedforward Loop to Upregulate SREBP1 through Sp1 O-GlcNAcylation and Augments Lipid Droplet Formation in Cancer Cells[J]. International journal of molecular sciences, 2021, 22(18): 9814.

Background

BODIPY 505/515은 지방 친화성 형광 염료로, 지질 드롭렛 라벨링에 광범위하게 사용되며 (Ex/Em: 505/515 nm) [1,2], 에피형광 현미경, 콘포컬 현미경 및 이차원 광학 현미경, 흐름세포측정기와 호환되며 생체 내외에서 사용할 수 있습니다. Bodipy 505/515는 인지질, 지방산, 콜레스테릴 에스터, 콜레스테롤 및 세라마이드 등 다양한 유형의 지질을 라벨링하는 데 사용됩니다.

References:
[1]. Velmurugan, N., Sathishkumar, Y., Yim, S.S., et al. Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101. Bioresour. Technol. 161, 149-154 (2014).
[2]. Rumin, J., Bonnefond, H., Saint-Jean, B., et al. The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae. Biotechnol. Biofuels 8, 42 (2015).
[3]. Elle I C, Olsen L C B, Pultz D, et al. Something worth dyeing for: molecular tools for the dissection of lipid metabolism in Caenorhabditis elegans[J]. FEBS letters, 2010, 584(11): 2183-2193.

Chemical Properties

Cas No. 21658-70-8 SDF
Chemical Name (T-4)-[2-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron
Canonical SMILES [F-][B+3]([N-]1C2=C(C)C=C1C)([N](C(C(C)=C3)=C2)=C3C)[F-]
Formula C13H15BF2N2 M.Wt 248.1
Solubility 1.7mg/ml in DMSO; 25mg/ml in DMF Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.0306 mL 20.1532 mL 40.3063 mL
5 mM 0.8061 mL 4.0306 mL 8.0613 mL
10 mM 0.4031 mL 2.0153 mL 4.0306 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

리뷰

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Average Rating: 5 ★★★★★ (Based on Reviews and 10 reference(s) in Google Scholar.)

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