>>Signaling Pathways>> Cell Cycle/Checkpoint>> Proteolysis>>Ac-VDVAD-AFC

Ac-VDVAD-AFC (Synonyms: N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin Caspase-2 Substrate (Fluorogenic))

Catalog No.GC13400

Ac-VDVAD-AFC는 카스파제 특이적 형광 기질입니다. Ac-VDVAD-AFC는 caspase-3-like 활성 및 caspase-2 활성을 측정할 수 있으며 종양 및 암 연구에 사용할 수 있습니다.

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Ac-VDVAD-AFC Chemical Structure

Cas No.: 210344-94-8

Size 가격 재고 수량
1mg
US$106.00
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5mg
US$288.00
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10mg
US$524.00
재고 있음

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Sample solution is provided at 25 µL, 10mM.

Description Chemical Properties Product Documents Related Products

Ac-VDVAD-AFC is a fluorogenic substrate whose amino acids VDVAD have been shown to be a preferred cleavage site for caspase-2.1,2 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. Increases in Ac-VDVAD-AFC cleavage have been reported to correlate with nitric oxide-induced activation of caspase-2 and subsequent apoptosis in human neuroblastoma lines.1

Product Description References

1. Moriya, R., Uehara, T., and Nomura, Y. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484(3), 253-260 (2000).

2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).

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