DLinDMA (Synonyms: 1,2-Dilinoleyloxy-N,N-dimethyl-3-aminopropane) |
Catalog No.GC33476
One-Click Copy Product Info
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DLinDMA는 안정적으로 핵산 지질 입자의 핵심 지질 성분으로 기준 물질로 사용된다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 871258-12-7
Sample solution is provided at 25 µL, 10mM.
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DLinDMA는 안정적으로 핵산 지질 입자의 핵심 지질 성분으로 기준 물질로 사용된다. DLinDMA는 siRNA 전달에 사용할 수 있다[1]. DLinDMA (1.56-25µM; 48시간)은 HEK293 세포와 SK-N-SH 세포에 독성을 나타내지 않았다[2]. DLinDMA (25-200µM; 48시간)은 용량 의존적으로 HEK293 세포와 SK-N-SH 세포의 증식을 억제하였다[2]. DLinDMA를 함유한 LNPs는 에스터 결합이 부족해서 지질효소에 의한 분해에 덜 취약하므로 더 효율적인 유전자 침묵을 달성한다[3]. 핵산 지질 과립 (SNALP)의 핵심 지질 성분으로서 DLinDMA는 쥐에서 소간섭 RNA (siRNA)를 전달할 수 있다[1].
이 계획은 문헌을 기반으로 해서 특정 요구에 맞게 수정하시기 바랍니다.
DLinDMA 리포솜 제조[1]
1)DLinDMA (5mM), DOPC (10mM), DOPE (2mM) 및 콜레스테롤 (2mM)을 에탄올에 용해시켰습니다.
2)생성된 유기상을 수상층 (Milli-Q 물)에 주입해서 리포솜이 자발적으로 형성되도록 하였습니다.
3)리포솜 소생된 용액을 30 진폭에서 120초 동안 프로브 초음파 처리해서 나노리포솜을 얻었습니다.
4)리포솜을 실온에서 교반해서 에탄올 잔류물을 제거하였습니다.
| Cas No. | 871258-12-7 | SDF | |
| Synonyms | 1,2-Dilinoleyloxy-N,N-dimethyl-3-aminopropane | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\CCCCCCCCOCC(OCCCCCCCC/C=C\C/C=C\CCCCC)CN(C)C | ||
| Formula | C41H77NO2 | M.Wt | 616.06 |
| Solubility | Ethanol : ≥ 100 mg/mL (162.32 mM);DMSO : < 1 mg/mL (insoluble or slightly soluble) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6232 mL | 8.1161 mL | 16.2322 mL |
| 5 mM | 324.6 μL | 1.6232 mL | 3.2464 mL |
| 10 mM | 162.3 μL | 811.6 μL | 1.6232 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)