Glucose oxidase(100-250u/mg) |
Catalog No.GC19780
One-Click Copy Product Info
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Glucose oxidase(100-250u/mg)은 누룩곰팡이에서 유래한 산화환원효소이다. 산소와 포도당이 존재할 때 Glucoamylase(100-250u/mg)은 항균 활성을 보일 수 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9001-37-0
Sample solution is provided at 25 µL, 10mM.
Glucose oxidase(100-250u/mg)은 누룩곰팡이에서 유래한 산화환원효소이다. 산소와 포도당이 존재할 때 Glucoamylase(100-250u/mg)은 항균 활성을 보일 수 있다.
Glucoamylase(100-250u/mg)은 누룩곰팡이에서 유래한 노란색 비정질 분말이다. 효소 커미션 번호는 EC 1.1.3.4이다. 특정 조건(pH 5.7, 37°C)에서 한 단위의 Glucoamylase는 분당 1μM 포도당을 산화시킬 수 있다. 효소의 분자량은 SDS-PAGE에 의해 결정된 약 150 kDa이다. 등전점는 5.2로 일정한 전하 특성을 보인다.
기질 D-포도당에 대해 효소의 미칼리스 상수는 4.9×10⁻² M이다. 실제 측정 결과 제품의 pH 안정성은 5.5~7.5(25°C, 18시간)이고 열 안정성은 <40°C(pH 5.7, 60분)이다. 주목할 만한 점은 Cu²⁺, Fe³⁺, Mg²⁺, Mn²⁺ 등과 같은 금속 이온이 Glucose oxidase(100-250u/mg)에 대한 억제 효과가 있다는 것이다. Glucose oxidase(100-250u/mg)는 β-D-pyranose 포도당에 특이적으로 결합하고 α-D-포도당에는 작용하지 않는다. 공기와 포도당이 존재할 때 Glucose oxidase(100-250u/mg)는 β-D-포도당의 산화를 촉매하고 D-glucono-1,5-lactone을 생성하고 글루콘산으로 수분해된다[4]. Glucose oxidase(100-250u/mg)는 혈당 모니터링, 식물 원료 및 식품 산업에서의 자유 포도당 검출, 식품 보존 등에 널리 사용된다. 게다가 생물 기술 분야에서도 많은 응용이 있고 일반적으로 생화학 분야의 효소 분석, 나노 기술 분야의 바이오센서 등에 사용된다[5].
References:
[1].Wong C M, Wong K H, Chen X D. Glucose oxidase: natural occurrence, function, properties and industrial applications[J]. Applied microbiology and biotechnology, 2008, 78: 927-938.
[2].Broom W A, Coulthard C E, Gurd M R, et al. Some pharmacological and chemotherapeutic properties of notatin[J]. British journal of pharmacology and chemotherapy, 1946, 1(4): 225.
[3].Ghoshdastider U, Wu R, Trzaskowski B, et al. Molecular effects of encapsulation of glucose oxidase dimer by graphene[J]. RSC Advances, 2015, 5(18): 13570-13578.
[4]. Bauer J A, Zámocká M, Majtán J, et al. Glucose oxidase, an enzyme “Ferrari”: Its structure, function, production and properties in the light of various industrial and biotechnological applications[J]. Biomolecules, 2022, 12(3): 472.
[5].Heller A, Ulstrup J. Detlev Müller’s Discovery of Glucose Oxidase in 1925[J]. Analytical Chemistry, 2021, 93(18): 7148-7149.
| Cas No. | 9001-37-0 | SDF | |
| Formula | M.Wt | approx 150 kDa | |
| Solubility | Soluble in water | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 212u/mg solid Appearance: Yellowish powder, lyophilized.
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)