KH 7 |
Catalog No.GC16334
One-Click Copy Product Info
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KH 7은 재조합 sAC 단백질에 대해 IC50이 약 3-10µM인 가용성 아데닐산 사이클라제(sAC)의 특이적 억제제이다.
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Cas No.: 330676-02-3
Sample solution is provided at 25 µL, 10mM.
KH 7은 재조합 sAC 단백질에 대해 IC50이 약 3-10µM인 가용성 아데닐산 사이클라제(sAC)의 특이적 억제제이다. 이 화합물은 정자 운동성, 선립선체 기능 및 세포 내 cAMP 신호전달 연구에서 일반적으로 사용된다[2].
KH 7(10-50μM; 1시간)을 야생형 정자에 추가하면 KH7 농도의 IC50이 10μM일 때 배양 유도된 cAMP 수준 증가를 억제하였다. 더 높은 농도(50μM)에서 KH 7은 그 IC50보다 5-10배 높은 농도에도 불구하고 sAC에 선택적이었다(하지만 tmAC에는 그렇지 않았다)고 해서 정자에서 기초 cAMP 축적을 현저하게 감소시켰다[1]. KH 7로 처리된 섬유아세포 배양은 세포 내 미토콘드리아에서의 cAMP 생성을 억제해서 핵 유전자에서 부터 유래된 복합체 I의 성숙 단위 NDUFS4, NDUFV2, NDUFA9의 총량을 상당히(35-50%) 감소시켰다[2]. 인간 탯줄 정맥 내피세포(HUVECs)에 KH 7(10µM)을 1시간 동안 처리하면 내피세포에서 세포 cAMP 수준을 현저하게 감소시켰다[3]. sAC를 안정적으로 과발현하는 293T-HEK 세포에서 KH 7(25μM; 1시간)을 처리하면 향상된 호흡, 증가된 COX 활성, 상승된 ATP 합성을 현저하게 억제하였다[4].
References:
[1]. De Rasmo D, Signorile A, Santeramo A, et al. Intramitochondrial adenylyl cyclase controls the turnover of nuclear-encoded subunits and activity of mammalian complex I of the respiratory chain[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2015, 1853(1): 183-191.
[2]. Chen M, Yi L, Jin X, et al. Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway[J]. Autophagy, 2013, 9(12): 2033-2045.
[3]. Hess K C, Jones B H, Marquez B, et al. The “soluble” adenylyl cyclase in sperm mediates multiple signaling events required for fertilization[J]. Developmental cell, 2005, 9(2): 249-259.
[4]. Acin-Perez R, Salazar E, Kamenetsky M, et al. Cyclic AMP produced inside mitochondria regulates oxidative phosphorylation[J]. Cell metabolism, 2009, 9(3): 265-276.
| 세포 실험 [1]: | |
세포 라인 | 인간 섬유아세포 |
제조 방법 | 섬유아세포는 10% 태아 소 혈청이 첨가된 DMEM에서 배양되었고 각각 1μM 이소프로테레놀과 25μM KH 7로 35분 동안 처리하였습니다. 인간 섬유아세포 배양에서 전체 세포와 미토콘드리아 외막 수준의 cAMP 수준에 대한 KH 7과 이소프로테레놀의 영향을 조사하였습니다. |
반응 조건 | 25μM; 35 분 동안 |
응용 분야 | KH 7로 처리된 섬유아세포 배양은 세포 내 미토콘드리아에서의 cAMP 생성을 억제해서 핵 유전자에서 부터 유래된 복합체 I의 성숙 단위 NDUFS4, NDUFV2, NDUFA9의 총량을 상당하게(35-50%) 감소시켰습니다. |
References: | |
| Cas No. | 330676-02-3 | SDF | |
| Chemical Name | (S,Z)-2-((1H-benzo[d]imidazol-2-yl)thio)-N'-((3-bromo-6-oxocyclohexa-2,4-dien-1-ylidene)methyl)propanehydrazide | ||
| Canonical SMILES | BrC1=C/C(C(C=C1)=O)=C/NNC([C@H](C)SC2=NC3=CC=CC=C3N2)=O | ||
| Formula | C17H15BrN4O2S | M.Wt | 419.3 |
| Solubility | 25 mg/ml in DMSO, 30 mg/ml in DMF, 0.5 mg/ml in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3849 mL | 11.9246 mL | 23.8493 mL |
| 5 mM | 477 μL | 2.3849 mL | 4.7699 mL |
| 10 mM | 238.5 μL | 1.1925 mL | 2.3849 mL |
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Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)