KH 7 |
Catalog No.GC16334
One-Click Copy Product Info
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KH 7 is a specific inhibitor of soluble adenylyl cyclase (sAC) with an IC50 of approximately 3-10µM against recombinant sAC protein .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 330676-02-3
Sample solution is provided at 25 µL, 10mM.
KH 7 is a specific inhibitor of soluble adenylyl cyclase (sAC) with an IC50 of approximately 3-10µM against recombinant sAC protein [1]. This compound is commonly used in studies of sperm motility, mitochondrial function, and intracellular cAMP signaling [2].
KH 7 (10-50μM; 1h) was added to wild-type sperm and inhibited the capacitation-induced increase in cAMP levels at a KH7 concentration with an IC50 of 10μM. At a higher concentration (50μM), KH 7 was selective for sAC (but not tmAC), despite concentrations 5- to 10-fold higher than its IC50, and significantly reduced basal cAMP accumulation in sperm[1]. Treatment of fibroblast cultures with KH 7, which inhibits intramitochondrial production of cAMP, causes a marked (35-50%) decrease of the overall amount of the mature forms of the nuclear-encoded NDUFS4, NDUFV2 and NDUFA9 subunits of complex I [2]. Treatment of human umbilical vein endothelial cells (HUVECs) with KH 7 (10µM) for 1 hour significantly reduced cellular cAMP levels in the endothelial cells [3]. In 293T-HEK cells stably overexpressing sAC and treated with KH 7(25μM; 1h), there was significant inhibition of enhanced respiration, increased COX activity, and elevated ATP synthesis[4].
References:
[1]. De Rasmo D, Signorile A, Santeramo A, et al. Intramitochondrial adenylyl cyclase controls the turnover of nuclear-encoded subunits and activity of mammalian complex I of the respiratory chain[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2015, 1853(1): 183-191.
[2]. Chen M, Yi L, Jin X, et al. Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway[J]. Autophagy, 2013, 9(12): 2033-2045.
[3]. Hess K C, Jones B H, Marquez B, et al. The “soluble” adenylyl cyclase in sperm mediates multiple signaling events required for fertilization[J]. Developmental cell, 2005, 9(2): 249-259.
[4]. Acin-Perez R, Salazar E, Kamenetsky M, et al. Cyclic AMP produced inside mitochondria regulates oxidative phosphorylation[J]. Cell metabolism, 2009, 9(3): 265-276.
| Cell experiment [1]: | |
Cell lines | Human fibroblast cell |
Preparation Method | Fibroblasts were grown in DMEM supplemented with 10% fetal bovine serum and treated with 1μM isoproterenol and 25μM KH 7 for 35 minutes, respectively. The effects of KH 7 and isoproterenol on cAMP levels in the whole cell and at the outer mitochondrial membrane level in human fibroblast cultures were investigated. |
Reaction Conditions | 25μM; 35min |
Applications | Treatment of fibroblast cultures withKH 7, which inhibits intramitochondrial production of cAMP, causes a marked (35–50%) decrease of the overall amount of the mature forms of the nuclear-encoded NDUFS4, NDUFV2 and NDUFA9 subunits of complex I. |
References: | |
| Cas No. | 330676-02-3 | SDF | |
| Chemical Name | (S,Z)-2-((1H-benzo[d]imidazol-2-yl)thio)-N'-((3-bromo-6-oxocyclohexa-2,4-dien-1-ylidene)methyl)propanehydrazide | ||
| Canonical SMILES | BrC1=C/C(C(C=C1)=O)=C/NNC([C@H](C)SC2=NC3=CC=CC=C3N2)=O | ||
| Formula | C17H15BrN4O2S | M.Wt | 419.3 |
| Solubility | 25 mg/ml in DMSO, 30 mg/ml in DMF, 0.5 mg/ml in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3849 mL | 11.9246 mL | 23.8493 mL |
| 5 mM | 477 μL | 2.3849 mL | 4.7699 mL |
| 10 mM | 238.5 μL | 1.1925 mL | 2.3849 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)