Melatonin (Synonyms: NSC 56423, NSC 113928, Regulin) |
| Catalog No.GC15618 |
Melatonin은 소뇌상체에서 생성되는 호르몬으로 Melatonin 수용체를 활성화할 수 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 73-31-4
Sample solution is provided at 25 µL, 10mM.
Melatonin은 소뇌상체에서 생성되는 호르몬으로 Melatonin 수용체를 활성화할 수 있다. Melatonin은 수면에 작용하고 중요한 항산화 및 항염증 특성을 가지고 있다[1][2][3]. Melatonin은 새로운 선택적 ATF-6 억제제이고 COX-2 저하를 통해 인간 간암 세포 사멸을 유도한다[4].
Melatonin은 동물에서 활성화된 PTEN, RSK-1, mTOR 및 AMPKα 키나아제 수준을 증가시키고 ERK-1/2 인산화와 Bad 인산화를 약하게 억제하고 S6 표준의 단백질, 4E-BP1, GSK-3α 및 GSK-3β의 인산화를 현저하게 억제하고 PRAS40 인산화를 약간 증가시킨다[1]. Melatonin은 대뇌 피질에서 Aβ1-42가 유도하는 신경독성 및 아스트로사이트 활성을 개선한다. Melatonin은 또한 Aβ1-42가 유도하는 Reelin과 Dab1 발현의 감소를 차단한다[2]. Melatonin 처리와 NLRP3-/- 결핍은 쥐에서 NF-κB 및 NLRP3 신호전달 경로를 현저하게 억제한다. Melatonin 처리와 NLRP3-/- 결핍은 시계 유전자 발현 패턴을 공유하고 쥐 심근세포의 형태를 개선한다[3].
References:
[1]. Kilic U, et al. Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice. Redox Biol. 2017 Apr 5;12:657-665
[2]. Hu C, et al. Neuroprotective effect of melatonin on soluble Aβ1-42-induced cortical neurodegeneration via Reelin-Dab1 signaling pathway. Neurol Res. 2017 Apr 7:1-1
[3]. Rahim I, et al. Melatonin administration to wild-type mice and non-treated NLRP3 mutant mice share similar inhibition of the inflammatory response during sepsis. J Pineal Res. 2017 Mar 31
[4]. Bu LJ, et al. Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation. World J Gastroenterol. 2017 Feb 14;23(6):986-998.
| 동물 실험 []: | |
동물 실험 | 21-26 g의 체중을 가진 성체 수컷 C57BL/6j 쥐 두 그룹을 네 그룹 중 하나로 무작위로 배정해서 (i) 대차물질 (50 µL isotonic saline /5% ethanol), (ii) ethanol (4 mg/kg, 0.9% isotonic saline /5% ethanol에 녹인), (iii) Wortmanni 그리고 (iv) melatonin/Wortmannin을 재관류 후 즉시 복강내 투여하였습니다. 첫 번째 그룹에서는 쥐를 30분 동안의 초점성 뇌허혈 (FCI) 과 72시간 재관류에 노출시켜 문상체의 분산된 허혈 손상을 평가하고 신호전달 경로를 분석하였습니다 (그룹당 n=7). 두 번째 쥐 그룹은 90분 동안의 FCI와 24시간 재관류에 노출되어 뇌졸중 발달, 뇌 부종 및 IgG 유출을 분석하였습니다 (그룹당 n=7). |
References: [1]. Kilic U, et al. Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice. Redox Biol. 2017 Apr 5;12:657-665 | |
| Cas No. | 73-31-4 | SDF | |
| Synonyms | NSC 56423, NSC 113928, Regulin | ||
| Chemical Name | N-[2-(5-methoxy-1H-indol-3-yl)ethyl]acetamide | ||
| Canonical SMILES | CC(=O)NCCC1=CNC2=C1C=C(C=C2)OC | ||
| Formula | C13H16N2O2 | M.Wt | 232.28 |
| Solubility | ≥ 100mg/mL in DMSO; ≥ 100mg/mL in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.3051 mL | 21.5257 mL | 43.0515 mL |
| 5 mM | 861 μL | 4.3051 mL | 8.6103 mL |
| 10 mM | 430.5 μL | 2.1526 mL | 4.3051 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 32 reference(s) in Google Scholar.)
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