NSC 3852 |
| Catalog No.GC15040 |
A tumor cell differentiating agent
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3565-26-2
Sample solution is provided at 25 µL, 10mM.
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NSC 3852 is a potent inhibitor of histone deacetylase [1].
Histone deacetylases (HADC) are a series of enzymes that remove acetyl groups from an ε-N-acetyl lysine amino acid on a histone and make the histones to wrap the DNA more tightly, which prevent transcription.
In MCF-7 human breast cancer cells, NSC 3852 induced the formation of the DMPO-OH adduct, which was catalyzed by superoxide dismutase. The result suggested that NSC 3852 induced generation of superoxide and reactive oxygen species (ROS), which produced DNA damage and induced apoptosis. Also, NSC 3852 resulted in cells accumulation in G0, hypophosphorylated retinoblastoma protein(Rb) and reduced E2F1 and Myc protein [1]. In HS68 cell, NSC 3852 inhibited Toxoplasma gondii tachyzoite propagation with EC50 value of 78.6 nM. Also, in human red blood cells, NSC 3852 inhibited Plasmodium falciparum growth with EC50 value of 1.3 μM. NSC 3852 inhibited Toxoplasma gondii tachyzoite propagation by involving redox reactions [2].
References:
[1]. Martirosyan A, Leonard S, Shi X, et al. Actions of a histone deacetylase inhibitor NSC3852 (5-nitroso-8-quinolinol) link reactive oxygen species to cell differentiation and apoptosis in MCF-7 human mammary tumor cells. J Pharmacol Exp Ther, 2006, 317(2): 546-552.
[2]. Strobl JS, Seibert CW, Li Y, et al. Inhibition of Toxoplasma gondii and Plasmodium falciparum infections in vitro by NSC3852, a redox active antiproliferative and tumor cell differentiation agent. J Parasitol, 2009, 95(1): 215-223.
| Cas No. | 3565-26-2 | SDF | |
| Chemical Name | 5-nitrosoquinolin-8-ol | ||
| Canonical SMILES | C1=CC2=C(C=CC(=C2N=C1)O)N=O | ||
| Formula | C9H6N2O2 | M.Wt | 174.16 |
| Solubility | ≥ 7.35mg/mL in DMSO | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 5.7418 mL | 28.7092 mL | 57.4185 mL |
| 5 mM | 1.1484 mL | 5.7418 mL | 11.4837 mL |
| 10 mM | 574.2 μL | 2.8709 mL | 5.7418 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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