Tunicamycin Mixture |
Catalog No.GC16738
One-Click Copy Product Info
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Tunicamycin Mixture은 동종인 핵간류 항생제의 혼합물로, N-당질화를 억제하고 GlcNAc 인산전달효소(GPT)를 차단한다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11089-65-9
Sample solution is provided at 25 µL, 10mM.
Tunicamycin Mixture은 동종인 핵간류 항생제의 혼합물로, N-당질화를 억제하고 GlcNAc 인산전달효소(GPT)를 차단한다.
Tunicamycin Mixture은 세포 내 지방질망(ER) 스트레스를 유도하고 DNA 합성 차단 및 G1 단계 정지를 유발한다[2]. Tunicamycin Mixture은 그람양성 세균, 엑스트라셀룰로스, 진균, 곰팡이 및 바이러스를 억제하고 항암활성이 있다[3].
체외 실험에서 RAW264.7 세포에 Tunicamycin Mixture(0.5μg/ml)을 2시간 동안 사전 처리한 것은 LPS 유도된 아질산염산생산을 현저하게 감소시켰으며, iNOS와 COX-2 mRNA 및 단백질의 표현을 약화시켰다[4]. Tunicamycin Mixture(1-20μg/ml)은 24-96시간 동안 전립선 암 세포 PC-3에 대해 처리하고, 용량에 따라 세포 활성을 감소시켰으며, 단백질의 N-당질화 형성을 차단하고 내 지방질망 스트레스를 유도했다[5].
체내 실험에서 Tunicamycin Mixture(1mg/kg)을 구강 내 복용으로 주입하여 쥐에게, 간의 트리글리세라이드 축적을 현저하게 증가시켰으며 간의 혈지 단백질 비밀도를 억제하고, 혈당 수준과 간의 글루코간 함량을 감소시켰다[6]. 중성 백혈구성 천식 모델(OVALPS-OVA 쥐)에서 기도 내 투여로 Tunicamycin Mixture (2μg)을 투여함으로써, 내 지방질망 스트레스 표지와 염증성 인자 사이토카인의 단백질 표현 수준을 증가시켜, 쥐의 천식 현상이 더욱 심각해졌다[7].
References:
[1] Hsu J L, Chiang P C, Guh J H. Tunicamycin induces resistance to camptothecin and etoposide in human hepatocellular carcinoma cells: role of cell-cycle arrest and GRP78[J]. Naunyn-Schmiedeberg's archives of pharmacology, 2009, 380: 373-382.
[2] Han C, Jin L, Mei Y, et al. Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells[J]. Cellular signalling, 2013, 25(1): 144-149.
[3] Pałasz A, Cież D. In search of uracil derivatives as bioactive agents. Uracils and fused uracils: Synthesis, biological activity and applications[J]. European journal of medicinal chemistry, 2015, 97: 582-611.
[4] Kim S Y, Hwang J S, Han I O. Tunicamycin inhibits Toll-like receptor-activated inflammation in RAW264. 7 cells by suppression of NF-κB and c-Jun activity via a mechanism that is independent of ER-stress and N-glycosylation[J]. European journal of pharmacology, 2013, 721(1-3): 294-300.
[5] Guha P, Kaptan E, Gade P, et al. Tunicamycin induced endoplasmic reticulum stress promotes apoptosis of prostate cancer cells by activating mTORC1[J]. Oncotarget, 2017, 8(40): 68191.
[6] Feng B, Huang X, Jiang D, et al. Endoplasmic reticulum stress inducer tunicamycin alters hepatic energy homeostasis in mice[J]. International journal of molecular sciences, 2017, 18(8): 1710.
[7]Guo Q, Li H, Liu J, et al. Tunicamycin aggravates endoplasmic reticulum stress and airway inflammation via PERK-ATF4-CHOP signaling in a murine model of neutrophilic asthma[J]. Journal of Asthma, 2017, 54(2): 125-133.
| 세포 실험[1]: | |
세포 라인 | RAW264.7 세포 |
제조 방법 | RAW264.7 세포에 0.5μg/ml의 Tunicamycin Mixture를 2시간 동안 사전 처리한 다음, LPS와 함께 18시간 동안 배양하였다. |
반응 조건 | 0.5μg/ml; 2 h |
응용 분야 | Tunicamycin Mixture은 LPS가 유도한 아질산염산의 생산/방출을 현저하게 감소시켰으며, iNOS와 COX-2 mRNA 및 단백질 표현을 완화시켰다. |
동물 실험 [2]: | |
동물 모형 | C57BL/6 수컷 쥐 |
제조 방법 | 쥐는 비슷한 평균 체중과 혈당 수준에 따라 무작위로 2 그룹으로 나뉘었다. 한 그룹은 1mg/kg Tunicamycin Mixture 또는 배달체를 구강 내 복용으로 주입받았다. 주입 후 24시간 동안 밥을 먹은 상태에서 체중과 혈당 수준을 측정했다. 그 후, 쥐는 이산화탄소를 사용하여 마취살해를 실시한 다음 자궁경부 탈구을 진행했다. |
제형 | 1mg/kg；i.p. |
응용 분야 | Tunicamycin Mixture 24시간 처리는 간의 트리글리세라이드 축적을 현저하게 증가시켰으며, 간의 혈지 단백질 비밀도를 억제하고, 혈당 수준과 간의 글루코간 함량을 감소시켰다. |
참고문헌: [1] Kim S Y, Hwang J S, Han I O. Tunicamycin은 ER 스트레스와 N-당질화에 독립적인 메커니즘을 통해 NF-κB와 c-Jun 활성을 억제하여 RAW264.7 세포에서 톨样 수용체 활성화 염증을 억제한다[J]. 유럽 약리학 저널, 2013, 721(1-3): 294-300. [2] 풍 B, 황 X, 장 D 등.내 지방질망 스트레스 유도체 튜니카마이신은 쥐 간 에너지 역학 균형을 변화시킨다[J]. 국제 분자 과학 저널, 2017, 18(8): 1710. | |
| Cas No. | 11089-65-9 | SDF | |
| Formula | C39H64N4O16 (for Tunicamycin VII) | M.Wt | 845.0 |
| Solubility | DMF: 20 mg/ml DMSO: 20 mg/ml DMSO:PBS(pH 7.2) (1:3): 0.25 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1834 mL | 5.9172 mL | 11.8343 mL |
| 5 mM | 236.7 μL | 1.1834 mL | 2.3669 mL |
| 10 mM | 118.3 μL | 591.7 μL | 1.1834 mL |
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Note: 1. Please make sure the liquid is clear before adding the next solvent.
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- Purity: >95.00% Appearance: A solid
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Related Biological Data
Tibial and growth plate images. (A) Representative images of tibia. (B) Safranin O/fast green staining of tibial growth plate in the control, MPs + SAL, MPs, and MPs + TM groups.
The ER-stress agonist group was administered with MPs at 2.0 mg/kg.bw/d by gavage, followed by intraperitoneal injection of TM(GlpBio) at 100 mg/kg.bw/d after 30 min.
Environmental Toxicology (2024). PMID: 38440912 IF: 4.5003 -
Related Biological Data
Acacetin decreases the expression of lipid synthesis and ferroptosis-related genes, reduces iron overload, and inhibits oxidative stress. (A). Hepatic iron content. (B). The lipid peroxidation product MDA was tested. (C) Hepatic GSH was measured.
The decrease of intracellular TG and mRNA levels of genes related to lipid synthesis caused by acacetin was significantly reversed by erastin (5 mM) and TU (1 mM) (GlpBio)
Biochem Bioph Res Co 640 (2023): 183-191. PMID: 36516527 IF: 3.322 -
Related Biological Data
TREM1 knockdown reduces inflammation and apoptosis of NPCs through inhibiting ERS. (A) The levels of inflammatory factors were measured with ELISA kits. The influence of MPLA and tunicamycin on apoptosis of the NPCs was assessed using
Tunicamycin, an endoplasmic reticulum stress (ERS) agonist, was purchased from Glpbio Technology, Inc. and NPCs were treated with tunicamycin at a concentration at 5 µg/ml (20).
Exp Ther Med 24.4 (2022): 1-11. PMID: 36160910 IF: 2.7511
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)