Colcemid (Synonyms: Demecolcine, NSC 3096) |
| رقم الكتالوجGC40664 |
كولسيميد هو مثبط للهيكل الخلوي يسبب توقفًا ميتوزيًا في المرحلة G2/M أو توقفًا ميوتيًا في مرحلة تكسير الحويصلة (GVBD) في الخلايا الثديية أو البويضات، على التوالي.
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Cas No.: 477-30-5
Sample solution is provided at 25 µL, 10mM.
Colcemid is a cytoskeletal inhibitor that induces mitotic arrest in the G2/M phase or meiotic arrest in the vesicle rupture (GVBD) phase in mammalian cells or oocytes, respectively[1][2].Colchicine interferes with microtubule polymerization by tightly binding to tubulin dimers and prevents spindle microtubule formation by depolymerization [7].
Cell transformation was observed with doses which were non-cytotoxic and did not cause mitotic inhibition of the cells. Higher dose of colcemid (greater than 0.1 µg/ml) resulted in mitotic inhibition of the cells and a significant loss of colony forming ability, but no increase in the frequency of morphological transformation.A 14-fold increase in the number of aneuploid cells with a near diploid chromosome complement was found in cultures treated with 0.1 µg/ml colcemid and both chromosome loss and gain were induced[4]. Colcemid was more cytotoxic to cells in G2 + M than to G1 + S phase cells, and it slowed the progression of G1 cells to S. These effects of colcemid were much greater in aneuploid B16 melanoma cells than in pseudodiploid Chinese hamster ovary (CHO) cells[5]. Colcemid promotes UVC-induced apoptosis in Chinese hamster ovary cells (CHO.K1).Although colcemid did not affect the excision of UV-induced DNA damages such as photoproducts or cyclobutane pyrimidine dimers, colcemid accumulated the DNA breaks when it was added to cells following UV-irradiation[3].
A mitotic linear accumulation was obtained by continuous colcemid infusion at 5.82 µg/hr. Low dose colcemid infusion (0.582 and 1.455 µg/hr) for 14 hours did not accumulated mitotic cells, but doses more than 5.82 µg/hr of colcemid blocked it completely, accumulating 25.5% of cells after a 20 hours infusion[6].
References:
[1]. Tsuchida T, Yoshimura K, et,al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9. doi: 10.1002/(sici)1097-0320(19980401)31:43.0.co;2-i. PMID: 9551605.
[2]. Ashley M Rozario, Sam DuwÉ, et,al.Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy bioRxiv 2020.08.13.249664; doi:https://doi.org/10.1101/2020.08.13.249664
[3]. Li H, Chang TW, et,al. Colcemid inhibits the rejoining of the nucleotide excision repair of UVC-induced DNA damages in Chinese hamster ovary cells. Mutat Res. 2005 Dec 30;588(2):118-28. doi: 10.1016/j.mrgentox.2005.09.005. Epub 2005 Nov 11. PMID: 16290038.
[4]. Tsutsui T, Maizumi H, et,al. Colcemid-induced neoplastic transformation and aneuploidy in Syrian hamster embryo cells. Carcinogenesis. 1984 Jan;5(1):89-93. doi: 10.1093/carcin/5.1.89. PMID: 6690091.
[5]. Bhuyan BK, Adams EG, et,al. Colcemid effects on B16 melanoma cell progression and aberrant mitotic division. J Cell Physiol. 1987 Aug;132(2):237-45. doi: 10.1002/jcp.1041320207. PMID: 3624316.
[6]. Nomura T. [In vivo cell cycle synchronization of the murine sarcoma 180 by continuous colcemid infusion (author's transl)]. Nihon Seikeigeka Gakkai Zasshi. 1980 Dec;54(12):1719-32. Japanese. PMID: 7288228.
[7]. Rieder CL, Palazzo RE. Colcemid and the mitotic cycle. J Cell Sci. 1992 Jul;102 ( Pt 3):387-92. doi: 10.1242/jcs.102.3.387. PMID: 1506421.
| Cell experiment [1]: | |
Cell lines | Syrian hamster embryo cells |
Preparation Method | Treatment of Syrian hamster embryo cells in culture with 0.01-0.1 µg/ml of colcemid for 48 h resulted in morphological and neoplastic transformation of the cells. |
Reaction Conditions | 0.01-0.1 µg/ml of colcemid for 48 h |
Applications | Cell transformation was observed with doses which were non-cytotoxic and did not cause mitotic inhibition of the cells. Higher dose of colcemid (greater than 0.1 µg/ml) resulted in mitotic inhibition of the cells and a significant loss of colony forming ability, but no increase in the frequency of morphological transformation.A 14-fold increase in the number of aneuploid cells with a near diploid chromosome complement was found in cultures treated with 0.1 µg/ml colcemid and both chromosome loss and gain were induced. |
| Animal experiment [2]: | |
Animal models | Tumor bearing mice |
Preparation Method | Mice were injected intravenously with Colcemid |
Dosage form | 5.82 µg/hr for 4h |
Applications | A mitotic linear accumulation was obtained by continuous colcemid infusion at 5.82 µg/hr. Low dose colcemid infusion (0.582 and 1.455 µg/hr) for 14 hours did not accumulated mitotic cells, but doses more than 5.82 µg/hr of colcemid blocked it completely, accumulating 25.5% of cells after a 20 hours infusion. |
References: | |
| Cas No. | 477-30-5 | SDF | |
| المرادفات | Demecolcine, NSC 3096 | ||
| Chemical Name | (7S)-6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-benzo[a]heptalen-9(5H)-one | ||
| Canonical SMILES | COC1=CC=C2C([C@@H](NC)CCC3=C2C(OC)=C(OC)C(OC)=C3)=CC1=O | ||
| Formula | C21H25NO5 | M.Wt | 371.4 |
| الذوبان | 30mg/mL in ethanol, 25mg/mL in DMSO or DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6925 mL | 13.4626 mL | 26.9251 mL |
| 5 mM | 0.5385 mL | 2.6925 mL | 5.385 mL |
| 10 mM | 0.2693 mL | 1.3463 mL | 2.6925 mL |
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- Purity: >99.50%
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