Home>>Signaling Pathways>> Cell Cycle/Checkpoint>> Cytoskeleton & Motor Proteins>>Colcemid

Colcemid

Catalog No.GC40664

Colcemid Chemical Structure

An inhibitor of microtubule assembly

Size Price Stock Qty
1mg
$38.00
In stock
5mg
$95.00
In stock
10mg
$170.00
In stock
50mg
$753.00
In stock

Customer Reviews

Based on customer reviews.

Tel: (626) 353-8530 Email: sales@glpbio.com

Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

View current batch:

Background

Colcemid is a colchicine derivative that inhibits tubulin polymerization as potently as colchicine (IC50 = 2.1 and 2.4 μM, respectively) but is less toxic.[1],[2],[3] At very low (nanomolar) concentrations, colcemid suppresses microtubule dynamicity and inhibits cell migration, while at micromolar levels it blocks microtubule assembly, arresting cells in metaphase.[3],[4],[5] Mitotic block by colcemid is used to synchronize cells and for karyotyping in cytogenetic studies.[4],6 Prolonged exposure to colcemid can activate p53, leading to apoptosis.[7]

Reference:
[1]. Muzaffar, A., Brossi, A., Lin, C.M., et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J. Med. Chem. 33(2), 567-571 (1990).
[2]. Rieder, C.L., and Palazzo, R.E. Colcemid and the mitotic cycle. Journal of Cell Science 102(3), 387-392 (1992).
[3]. Jordan, M.A., and Wilson, L. Microtubules as a target for anticancer drugs. Nature Reviews.Cancer 4(4), 253-265 (2004).
[4]. Curlej, J., Bulla, J., and Chrenek, P. Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages. Zygote 18(3), 203-207 (2010).
[5]. Yang, H., Ganguly, A., and Cabral, F. Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs. The Journal of Biological Chemisty 285(42), 32242-32250 (2010).
[6]. Bayani, J., and Squire, J.A. Preparation of cytogenetic specimens from tissue samples. Current Protocols in Cell Biology 22(22.2), 1-15 (2004).
[7]. Sablina, A.A., Chumakov, P.M., Levine, A.J., et al. p53 activation in response to microtubule disruption is mediated by integrin-Erk signaling. Oncogene 20(8), 899-909 (2001).

Chemical Properties

Cas No. 477-30-5 SDF
Synonyms N/A
Chemical Name (7S)-6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-benzo[a]heptalen-9(5H)-one
Canonical SMILES COC1=CC=C2C([C@@H](NC)CCC3=C2C(OC)=C(OC)C(OC)=C3)=CC1=O
Formula C21H25NO5 M.Wt 371.4
Solubility 30mg/mL in ethanol, 25mg/mL in DMSO or DMF Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

Reviews

Review for Colcemid

Average Rating: 5 ★★★★★ (Based on Reviews and 29 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for Colcemid

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.