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22(S)-hydroxy Cholesterol (Synonyms: 22β-hydroxy Cholesterol)

Catalog No.GC18270

A synthetic oxysterol and LXR modulator

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22(S)-hydroxy Cholesterol Chemical Structure

Cas No.: 22348-64-7

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1mg
53,00 $US
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5mg
206,00 $US
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10mg
362,00 $US
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Sample solution is provided at 25 µL, 10mM.

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Background

22(S)-hydroxy Cholesterol is a synthetic oxysterol and a modulator of the liver X receptor (LXR). [1] t prevents monocyte chemoattractant protein 1 (MCP-1) expression induced by the LXR agonist GW 3965 in primary hepatocytes and downregulates mRNA expression of the LXR target genes CD36, ACSL1, and SCD-1 in human myotubes. It decreases triacylglycerol and diacylglycerol synthesis from labeled palmitate and acetate, respectively, in human myoblasts by 50% when used at a concentration of 10 uM. 22(S)-hydroxy Cholesterol also reduces fatty acid synthase (FAS) reporter activity through an LXR response element in the promoter region in COS-1 cells transfected with RXRα and LXRα and decreases the expression of MCP-1 and CCR2 in a mouse model of chronic ethanol consumption.[1] [2] Dietary supplementation of 22(S)-hydroxy cholesterol (30 mg/kg per day) leads to less body weight gain and lower liver triacylglycerol levels in rats when fed either a regular chow or high-fat diet as well as prevents an increase in plasma triacylglycerol levels resulting from a high-fat diet.[3]

Reference:
[1]. Kase, E.T., Andersen, B., Nebb, H.I., et al. 22-Hydroxycholesterols regulate lipid metabolism differently than T0901317 in human myotubes. Biochim. Biophys. Acta. 1761(12), 1515-1522 (2006).
[2]. Na, T.-Y., Han, Y.-H., Ka, N.-L., et al. 22-S-Hydroxycholesterol protects against ethanol-induced liver injury by blocking the auto/paracrine activation of MCP-1 mediated by LXRα. J. Pathol. 235(5), 710-720 (2015).
[3]. Kase, E.T., Nikolić, N., Pettersen Hessvik, N., et al. Dietary supplementation with 22-S-hydroxycholesterol to rats reduces body weight gain and the accumulation of liver triacylglycerol. Lipids 47(5), 483-493 (2012).

Chemical Properties

Cas No. 22348-64-7 SDF
Synonymes 22β-hydroxy Cholesterol
Chemical Name cholest-5-ene-3β,22S-diol
Canonical SMILES O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@]4([C@@H]([C@@H](O)CCC(C)C)C)[H]
Formula C27H46O2 M.Wt 402.7
Solubility 20mg/mL in Ethanol, 0.1mg/mL in DMSO, 2mg/mL in DMF, Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.4832 mL 12.4162 mL 24.8324 mL
5 mM 0.4966 mL 2.4832 mL 4.9665 mL
10 mM 0.2483 mL 1.2416 mL 2.4832 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 12 reference(s) in Google Scholar.)

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