CAM833 |
Catalog No.GC65935
One-Click Copy Product Info
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CAM833, un inhibiteur chimique sub-micromolaire de l'interaction protéine-protéine régulatrice entre la recombinase RAD51 et les motifs répétitifs BRC du suppresseur de tumeur BRCA2.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2758364-02-0
Sample solution is provided at 25 µL, 10mM.
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CAM833, un inhibiteur chimique sub-micromolaire de l'interaction protéine-protéine régulatrice entre la recombinase RAD51 et les motifs répétitifs BRC du suppresseur de tumeur BRCA2. CAM833 s'engage dans deux poches hydrophobes à la surface de RAD51 qui accueillent normalement les chaînes latérales hydrophobes conservées des répétitions BRC de BRCA2, entrant ainsi directement en compétition avec l'interaction RAD51:BRCA2. CAM833 supprime l'assemblage de RAD51 en filaments induits par les dommages, et entrave la réparation de l'ADN par recombinaison homologue de l'ADN. Le Kd de CAM833 inhibant ChimRAD51 est de 366 nM [1]. CAM833 potentialise l'inhibition de la croissance, l'arrêt du cycle cellulaire et la cytotoxicité induite par les lésions de l'ADN.
CAM833 (20 μM ; 0-72 h) a renforcé l'arrêt du cycle cellulaire induit par les radiations dans les cellules HCT116 et a augmenté l'apoptose au fil du temps, CAM833 a également renforcé l'inhibition de PARP1 dans les cellules HCT116 [1].
References:
[1]. Scott DE, Francis-Newton NJ, et,al. A small-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death. Cell Chem Biol. 2021 Jun 17;28(6):835-847.e5. doi: 10.1016/j.chembiol.2021.02.006. Epub 2021 Mar 3. PMID: 33662256; PMCID: PMC8219027.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellule HCT116 |
Méthode de préparation | Les cellules HCT116 ont été traitées avec 20 μM de CAM833 ou de DMSO, et l'exposition aux rayonnements ionisants de 3gy et le cycle cellulaire ont été analysés. |
Conditions de réaction | 20 μM；72h |
Domaines d'application | CAM833 renforce l'arrêt du cycle cellulaire induit par les radiations, augmente la progression des cellules arrêtées en G2/M vers l'apoptose dans les cellules HCT116. |
Références : | |
| Cas No. | 2758364-02-0 | SDF | |
| Formula | C26H26ClFN4O5 | M.Wt | 528.96 |
| Solubility | DMSO : 100 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8905 mL | 9.4525 mL | 18.905 mL |
| 5 mM | 378.1 μL | 1.8905 mL | 3.781 mL |
| 10 mM | 189.1 μL | 945.3 μL | 1.8905 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)