CAM833 |
Katalog-Nr.GC65935
One-Click Copy Product Info
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CAM833 ist ein potenter orthosterischer Inhibitor der Wechselwirkung zwischen BRCA2 und RAD51 mit einer Kd von 366 nM gegen das ChimRAD51-Protein. CAM833 hemmt auch die Oligomerisierung von RAD51.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2758364-02-0
Sample solution is provided at 25 µL, 10mM.
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CAM833, a sub-micromolar chemical inhibitor of the regulatory protein-protein interaction between the RAD51 recombinase and the BRC repeat motifs of the tumor suppressor BRCA2. CAM833 engages with two hydrophobic pockets on the surface of RAD51 that normally accommodate conserved hydrophobic side chains from the BRC repeats of BRCA2, thereby directly competing with the RAD51:BRCA2 interaction.CAM833 suppresses the assembly of RAD51 into damage-induced filaments, and impairs DNA repair by homologous DNA recombination. The Kd of CAM833 inhibiting ChimRAD51 is 366 nM [1]. CAM833 potentiates growth inhibition, cell-cycle arrest and cytotoxicity induced by DNA damage.
CAM833 (20 μM; 0-72 h) enhanced radiation-induced cell cycle arrest in HCT116 cells and increased apoptosis over time, CAM833 also enhanced the inhibition of PARP1 in HCT116 cells [1].
References:
[1]. Scott DE, Francis-Newton NJ, et,al. A small-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death. Cell Chem Biol. 2021 Jun 17;28(6):835-847.e5. doi: 10.1016/j.chembiol.2021.02.006. Epub 2021 Mar 3. PMID: 33662256; PMCID: PMC8219027.
| Cell experiment [1]: | |
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Cell lines |
HCT116 cell |
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Preparation Method |
HCT116 cells were treated with 20 μM CAM833 or DMSO, and exposure to 3gy ionizing radiation and the cell cycle was analyzed. |
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Reaction Conditions |
20 μM；72h |
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Applications |
CAM833 enhanced radiation-induced cell cycle arrest , increases the progression of G2/M-arrested cells into apoptosis in HCT116 cells. |
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References: [1]. Scott DE, Francis-Newton NJ, et,al. l-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death. Cell Chem Biol. 2021 Jun 17;28(6):835-847.e5. doi: 10.1016/j.chembiol.2021.02.006. Epub 2021 Mar 3. PMID: 33662256; PMCID: PMC8219027. |
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| Cas No. | 2758364-02-0 | SDF | |
| Formula | C26H26ClFN4O5 | M.Wt | 528.96 |
| Löslichkeit | DMSO : 100 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8905 mL | 9.4525 mL | 18.905 mL |
| 5 mM | 378.1 μL | 1.8905 mL | 3.781 mL |
| 10 mM | 189.1 μL | 945.3 μL | 1.8905 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)