Aurintricarboxylic Acid (ammonium salt) (Synonyms: ATA) |
| カタログ番号GC46895 |
多様な生物学的活性を持つタンパク質合成阻害剤
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 569-58-4
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Aurintricarboxylic acid (ATA) is an inhibitor of protein synthesis that has diverse biological activities.1,2,3,4,5,6,7,8 It inhibits the activity of a variety of enzymes, including the nucleases DNase I, RNase A, and S1, as well as the serine proteases trypsin and chymotrypsin in a concentration-dependent manner.2,3 ATA inhibits replication of severe acute respiratory syndrome coronavirus (SARS-CoV; EC50 = 200 µg/ml) and human enterovirus 71 in Vero cells (EV71; EC50 = 2.9 µM), as well as reduces HIV-1- or HIV-2-induced cytopathogenicity in MT-4 cells (IC50s = 1.1 and 0.85 µg/ml, respectively).4,5,6 It inhibits apoptosis induced by sanguinarine in K562 leukemia cells when used at a concentration of 100 µM.7 ATA (20 mg/kg, i.p.) decreases CNS CD4+ T cell infiltration and reduces hind limb weakness and paralysis in a mouse model of myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis (EAE).8
1.Siegelman, F., and Apirion, D.Aurintricarboxylic acid, a preferential inhibitor of initiation of protein synthesisJ. Bacteriol.105(3)902-907(1971) 2.Hallick, R.B., Chelm, B.K., Gray, P.W., et al.Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolationNucleic Acids Res.4(9)3055-3064(1977) 3.Bina-Stein, M., and Tritton, T.R.Aurintricarboxylic acid is a nonspecific enzyme inhibitorMol. Pharmacol.12(1)191-193(1976) 4.He, R., Adonov, A., Traykova-Adonova, M., et al.Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acidBiochem. Biophys. Res. Commun.320(4)1199-1203(2004) 5.Hung, H.-C., Chen, T.-C., Fang, M.-Y., et al.Inhibition of enterovirus 71 replication and the viral 3D polymerase by aurintricarboxylic acidJ. Antimicrob. Chemother.65(4)676-683(2010) 6.Cushman, M., Wang, P.L., Chang, S.H., et al.Preparation and anti-HIV activities of aurintricarboxylic acid fractions and analogues: Direct correlation of antiviral potency with molecular weightJ. Med. Chem.34(1)329-337(1991) 7.Hallock, S., Tang, S.-C., Buja, L.M., et al.Aurintricarboxylic acid inhibits protein synthesis independent, sanguinarine-induced apoptosis and oncosisToxicol. Pathol.35(2)300-309(2007) 8.Zhang, F., Wei, W., Chai, H., et al.Aurintricarboxylic acid ameliorates experimental autoimmune encephalomyelitis by blocking chemokine-mediated pathogenic cell migration and infiltrationJ. Immunol.190(3)1017-1025(2013)
| Cas No. | 569-58-4 | SDF | |
| 同義語 | ATA | ||
| Canonical SMILES | [O-]C(C(C(C=C/1)=O)=CC1=C(C2=CC=C(O)C(C([O-])=O)=C2)\C3=CC=C(O)C(C([O-])=O)=C3)=O.[NH4+].[NH4+].[NH4+] | ||
| Formula | C22H11O9.3NH4 | M.Wt | 473.4 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 100 μg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1124 mL | 10.5619 mL | 21.1238 mL |
| 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *