Chloroquine (Synonyms: CHLORO QUINE PHOSPHATE; 4-(7-chloro-4-quinolylamino)pentyldiethylamine; (4S)-N~4~-(7-chloroquinolin-4-yl)-N~1~,N~1~-diethylpentane-1,4-diamine) |
カタログ番号GC19549 |
クロロキンは、マラリアや関節リウマチの治療に広く使用されている抗マラリア薬および抗炎症薬です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 54-05-7
Sample solution is provided at 25 µL, 10mM.
- Autophagy (2024).PMID:38566321
- Cell Death Differ (2024):1-16.PMID:38418695
- Adv Healthc Mater (2023):2302691.PMID:37990414
- Clin. Transl. Med. 15.3 (2025):e70270.PMID:40088428
- Free Radical Bio Med (2024).PMID:39117049
- Ecotox Environ Safe 299 (2025):118374.PMID:40409189
- Cell Biol Toxicol 40.1 (2024):1-23.PMID:38267572
- Nutrients 14.8 (2022):1673.PMID:35458235
- Cns Neurosci Ther 31.2 (2025):e70305.PMID:40016173
- Cns Neurosci Ther 31.2 (2025):e70305.
- eGastroenterology 2.1 (2024).
- Front Cell Dev Biol 9 (2021):727822.PMID:34790659
Chloroquine is used as an antimalarial drug and also functions to increase sensitivity of tumor cells to radiation and chemotherapy via inducing autophagy [1].
Chloroquine has been reported as an adjuvant for radiation and chemotherapy for inducing cell autophagy to anti-cancer cells proliferation or metastasis [2]. The mechanism of chloroquine diphosphate inducing cells autophagy is arresting cells in G1, up-regulates the expression of p27 and p53 while down-regulates the expression of CDK2 and cyclin D1 [3].
Apart from anti-malarial, chloroquine also has long been reported functioning in cell apoptosis. Pretreated CNE-2 human nasopharyngeal carcinoma cells with chloroquine enhanced ionizing radiation induced cell apoptosis via increasing cells autophagic ratio [4]. When treated with mouse breast cancer 4T1 cells, chloroquine treatment inhibited cellular proliferation and viability which resulted in cells apoptosis in a time- and dose- dependent manner [2]. In human colon cancer DLD-1 cells, combination of 5-FU and chloroquine could inhibit cells proliferation via inducing autophagy [3].
In mouse model with 4T1 cells subcutaneous xenograft, chloroquine treatment significantly inhibited tumor growth and tumor cells metastasis to the lung, thus enhanced the mice survival [2]. In BALB/c mice injected with colon26 cells subcutaneously, chloroquine cooperated with 5-FU significantly enhanced the inhibition of tumor growth induced by 5-FU through increasing the ratio of apoptotic cells [5].
References:
[1]. Gewirtz, D.A., An autophagic switch in the response of tumor cells to radiation and chemotherapy. Biochem Pharmacol, 2014. 90(3): p. 208-11.
[2]. Jiang, P.D., et al., Antitumor and antimetastatic activities of chloroquine diphosphate in a murine model of breast cancer. Biomed Pharmacother, 2010. 64(9): p. 609-14.
[3]. Choi, J.H., et al., Chloroquine enhances the chemotherapeutic activity of 5-fluorouracil in a colon cancer cell line via cell cycle alteration. APMIS, 2012. 120(7): p. 597-604.
[4]. Zhou, Z.R., et al., Poly(ADP-ribose) polymerase-1 regulates the mechanism of irradiation-induced CNE-2 human nasopharyngeal carcinoma cell autophagy and inhibition of autophagy contributes to the radiation sensitization of CNE-2 cells. Oncol Rep, 2013. 29(6): p. 2498-506.
[5]. Sasaki, K., et al., Resistance of colon cancer to 5-fluorouracil may be overcome by combination with chloroquine, an in vivo study. Anticancer Drugs, 2012. 23(7): p. 675-82.
Cas No. | 54-05-7 | SDF | |
同義語 | CHLORO QUINE PHOSPHATE; 4-(7-chloro-4-quinolylamino)pentyldiethylamine; (4S)-N~4~-(7-chloroquinolin-4-yl)-N~1~,N~1~-diethylpentane-1,4-diamine | ||
Formula | C18H26ClN3 | M.Wt | 319.8721 |
溶解度 | 64 mg/mL (200.08 mM) in DMSO, 64 mg/mL (200.08 mM) in Ethanol, Insoluble in Water | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
1 mM | 3.1262 mL | 15.6312 mL | 31.2625 mL |
5 mM | 0.6252 mL | 3.1262 mL | 6.2525 mL |
10 mM | 0.3126 mL | 1.5631 mL | 3.1262 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
RABV-M blocks the autophagic flux. (A) RABV-M-FLAG was expressed in N2a cells for the indicated time periods, and cell lysates were analyzed by WB. (B) N2a cells were transfected with RABV-M-FLAG for 12 h and then treated with/without chloroquine (CQ, 2 μM) for 24 h. Cell lysates were analyzed by WB.
N2a cells were transfected with RABV-M-FLAG for 12 h and then treated with/without chloroquine (CQ, 2 μM, GlpBio) for 24 h.
Autophagy just-accepted (2024). PMID: 38566321 IF: 13.3003 -
Related Biological Data
Hypoxia-inducible factor-1α-mediated mitophagy prevented bevacizumab-induced ROS accumulation and apoptosis in vitro: Cultured 661W photoreceptors, MIO-M1 Müller cells, and HUVECs were treated with bevacizumab or CQ alone.
Chloroquine (10 mg/kg, GlpBio) was given 1 h prior to bevacizumab vitreous injection and then every 24 h until sacrifice.
Front Cell Dev Biol (2021): 3048. PMID: 34790659 IF: 6.684
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